LOCUS       sequence-106601-       13552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Filamin with internal stFRET inserted to measure 
            mechanical stress in real time.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13552)
  AUTHORS   Meng F, Suchyna TM, Sachs F
  TITLE     A fluorescence energy transfer-based mechanical stress sensor for 
            specific proteins in situ.
  JOURNAL   FEBS J. 2008 Jun;275(12):3072-87. doi: 
            10.1111/j.1742-4658.2008.06461.x. Epub 2008 May 10.
  PUBMED    18479457
REFERENCE   2  (bases 1 to 13552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1742-4658.2008.06461.x"; journalName: "FEBS J"; date: 
            "2008-06"; volume: "275"; issue: "12"; pages: "3072-87"
COMMENT     Sequence Label: Filamin-M-stFRET
FEATURES             Location/Qualifiers
     source          1..13552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(3627..3629,3630..4340)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4464..4466,4467..4469,4470..5180)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             5181..5204
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     polyA_signal    10340..10461
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10377..10396)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10431..10450
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(10468..10923)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10605..10626)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10817..10836
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10950..11054
                     /gene="bla"
                     /label=AmpR promoter
     promoter        11056..11413
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        11056..11247
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(11079..11099)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      11264..11399
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11448..12242
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             11448..12242
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11502..11521)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12112..12131
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(12430..12449)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    12474..12521
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      12850..13438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13339..13358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatga
      601 gtagctccca ctctcgggcg ggccagagcg cagcaggcgc ggctccgggc ggcggcgtcg
      661 acacgcggga cgccgagatg ccggccaccg agaaggacct ggcggaggac gcgccatgga
      721 agaagatcca gcagaacact ttcacgcgct ggtgcaacga gcacctgaag tgcgtgagca
      781 agcgcatcgc caacctgcag acggacctga gcgacgggct gcggcttatc gcgctgttgg
      841 aggtgctcag ccagaagaag atgcaccgca agcacaacca gcggcccact ttccgccaaa
      901 tgcagcttga gaacgtgtcg gtggcactcg agttcctgga ccgcgagagc atcaaactgg
      961 tgtccatcga cagcaaggcc atcgtggacg ggaacctgaa gctgatcctg ggcctcatct
     1021 ggaccctgat cctgcactac tccatctcca tgcccatgtg ggacgaggag gaggatgagg
     1081 aggccaagaa gcagaccccc aagcagaggc tcctgggctg gatccagaac aagctgccgc
     1141 agctgcccat caccaacttc agccgggact ggcagagcgg ccgggccctg ggcgccctgg
     1201 tggacagctg tgccccgggc ctgtgtcctg actgggactc ttgggacgcc agcaagcccg
     1261 ttaccaatgc gcgagaggcc atgcagcagg cggatgactg gctgggcatc ccccaggtga
     1321 tcacccccga ggagattgtg gaccccaacg tggacgagca ctctgtcatg acctacctgt
     1381 cccagttccc caaggccaag ctgaagccag gggctccctt gcggcccaaa ctgaacccga
     1441 agaaagcccg tgcctacggg ccaggcatcg agcccacagg caacatggtg aagaagcggg
     1501 cagagttcac tgtggagacc agaagtgctg gccagggaga ggtgctggtg tacgtggagg
     1561 acccggccgg acaccaggag gaggcaaaag tgaccgccaa taacgacaag aaccgcacct
     1621 tctccgtctg gtacgtcccc gaggtgacgg ggactcataa ggttactgtg ctctttgctg
     1681 gccagcacat cgccaagagc cccttcgagg tgtacgtgga taagtcacag ggtgacgcca
     1741 gcaaagtgac agcccaaggt cccggcctgg agcccagtgg caacatcgcc aacaagacca
     1801 cctactttga gatctttacg gcaggagctg gcacgggcga ggtcgaggtt gtgatccagg
     1861 accccatggg acagaagggc acggtagagc ctcagctgga ggcccggggc gacagcacat
     1921 accgctgcag ctaccagccc accatggagg gcgtccacac cgtgcacgtc acgtttgccg
     1981 gcgtgcccat ccctcgcagc ccctacactg tcactgttgg ccaagcctgt aacccgagtg
     2041 cctgccgggc ggttggccgg ggcctccagc ccaagggtgt gcgggtgaag gagacagctg
     2101 acttcaaggt gtacacaaag ggcgctggca gtggggagct gaaggtcacc gtgaagggcc
     2161 ccaagggaga ggagcgcgtg aagcagaagg acctggggga tggcgtgtat ggcttcgagt
     2221 attaccccat ggtccctgga acctatatcg tcaccatcac gtggggtggt cagaacatcg
     2281 ggcgcagtcc cttcgaagtg aaggtgggca ccgagtgtgg caatcagaag gtacgggcct
     2341 ggggccctgg gctggagggc ggcgtcgttg gcaagtcagc agactttgtg gtggaggcta
     2401 tcggggacga cgtgggcacg ctgggcttct cggtggaagg gccatcgcag gctaagatcg
     2461 aatgtgacga caagggcgac ggctcctgtg atgtgcgcta ctggccgcag gaggctggcg
     2521 agtatgccgt tcacgtgctg tgcaacagcg aagacatccg cctcagcccc ttcatggctg
     2581 acatccgtga cgcgccccag gacttccacc cagacagggt gaaggcacgt gggcctggat
     2641 tggagaagac aggtgtggcc gtcaacaagc cagcagagtt cacagtggat gccaagcacg
     2701 gtggcaaggc cccacttcgg gtccaagtcc aggacaatga aggctgccct gtggaggcgt
     2761 tggtcaagga caacggcaat ggcacttaca gctgctccta cgtgcccagg aagccggtga
     2821 agcacacagc catggtgtcc tggggaggcg tcagcatccc caacagcccc ttcagggtga
     2881 atgtgggagc tggcagccac cccaacaagg tcaaagtata cggccccgga gtagccaaga
     2941 cagggctcaa ggcccacgag cccacctact tcactgtgga ctgcgccgag gctggccagg
     3001 gggacgtcag catcggcatc aagtgtgccc ctggagtggt aggccccgcc gaagctgaca
     3061 tcgacttcga catcatccgc aatgacaatg acaccttcac ggtcaagtac acgccccggg
     3121 gggctggcag ctacaccatt atggtcctct ttgctgacca ggccacgccc accagcccca
     3181 tccgagtcaa ggtggagccc tctcatgacg ccagtaaggt gaaggccgag ggccctggcc
     3241 tcagtcgcac tggtgtcgag cttggcaagc ccacccactt cacagtaaat gccaaagctg
     3301 ctggcaaagg caagctggac gtccagttct caggactcac caagggggat gcagtgcgag
     3361 atgtggacat catcgaccac catgacaaca cctacacagt caagtacacg cctgtccagc
     3421 agggtccagt aggcgtcaat gtcacttatg gaggggatcc catccctaag agccctttct
     3481 cagtggcagt atctccaagc ctggacctca gcaagatcaa ggtgtctggc ctgggagaga
     3541 aggtggacgt tggcaaagac caggagttca cagtcaaatc aaagggtgct ggtggtaccg
     3601 gtggcggaaa gcttcagctg ggatccgtga gcaagggcga ggagctgttc accggggtgg
     3661 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     3721 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca
     3781 agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg cagtgcttcg
     3841 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3901 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3961 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     4021 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     4081 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     4141 aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     4201 ccgtgctgct gcccgacaac cactacctga gctaccagtc caaactgagc aaagacccca
     4261 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     4321 gcatggacga gctgtacaag tccggactca gatctgggct gcgcaagcgc ttacgaaaat
     4381 ttagaaacaa gattaaagaa aagcttaaaa aaattggtca gaaaatccag ggtttcgtgc
     4441 cgatacttgc aggtgtgaat tccatggtga gcaagggcga ggagctgttc accggggtgg
     4501 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     4561 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     4621 agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttcg
     4681 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     4741 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     4801 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     4861 aggacggcaa catcctgggg cacaagctgg agtacaacgc catcagcgac aacgtctata
     4921 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     4981 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     5041 ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca
     5101 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     5161 gcatggacga gctgtacaag catcatcacc accatcatca ccacggaggt ggcagcggcc
     5221 gccaaggcaa agtggcatcc aagattgtgg gcccctcggg tgcagcggtg ccctgcaagg
     5281 tggagccagg cctgggggct gacaacagtg tggtgcgctt cctgccccgt gaggaagggc
     5341 cctatgaggt ggaggtgacc tatgacggcg tgcccgtgcc tggcagcccc tttcctctgg
     5401 aagctgtggc ccccaccaag cctagcaagg tgaaggcgtt tgggccgggg ctgcagggag
     5461 gcagtgcggg ctcccccgcc cgcttcacca tcgacaccaa gggcgccggc acaggtggcc
     5521 tgggcctgac ggtggagggc ccctgtgagg cgcagctcga gtgcttggac aatggggatg
     5581 gcacatgttc cgtgtcctac gtgcccaccg agcccgggga ctacaacatc aacatcctct
     5641 tcgctgacac ccacatccct ggctccccat tcaaggccca cgtggttccc tgctttgacg
     5701 catccaaagt caagtgctca ggccccgggc tggagcgggc caccgctggg gaggtgggcc
     5761 aattccaagt ggactgctcg agcgcgggca gcgcggagct gaccattgag atctgctcgg
     5821 aggcggggct tccggccgag gtgtacatcc aggaccacgg tgatggcacg cacaccatta
     5881 cctacattcc cctctgcccc ggggcctaca ccgtcaccat caagtacggc ggccagcccg
     5941 tgcccaactt ccccagcaag ctgcaggtgg aacctgcggt ggacacttcc ggtgtccagt
     6001 gctatgggcc tggtattgag ggccagggtg tcttccgtga ggccaccact gagttcagtg
     6061 tggacgcccg ggctctgaca cagaccggag ggccgcacgt caaggcccgt gtggccaacc
     6121 cctcaggcaa cctgacggag acctacgttc aggaccgtgg cgatggcatg tacaaagtgg
     6181 agtacacgcc ttacgaggag ggactgcact ccgtggacgt gacctatgac ggcagtcccg
     6241 tgcccagcag ccccttccag gtgcccgtga ccgagggctg cgacccctcc cgggtgcgtg
     6301 tccacgggcc aggcatccaa agtggcacca ccaacaagcc caacaagttc actgtggaga
     6361 ccaggggagc tggcacgggc ggcctgggcc tggctgtaga gggcccctcc gaggccaaga
     6421 tgtcctgcat ggataacaag gacggcagct gctcggtcga gtacatccct tatgaggctg
     6481 gcacctacag cctcaacgtc acctatggtg gccatcaagt gccaggcagt cctttcaagg
     6541 tccctgtgca tgatgtgaca gatgcgtcca aggtcaagtg ctctgggccc ggcctgagcc
     6601 caggcatggt tcgtgccaac ctccctcagt ccttccaggt ggacacaagc aaggctggtg
     6661 tggccccatt gcaggtcaaa gtgcaagggc ccaaaggcct ggtggagcca gtggacgtgg
     6721 tagacaacgc tgatggcacc cagaccgtca attatgtgcc cagccgagaa gggccctaca
     6781 gcatctcagt actgtatgga gatgaagagg taccccggag ccccttcaag gtcaaggtgc
     6841 tgcctactca tgatgccagc aaggtgaagg ccagtggccc cgggctcaac accactggcg
     6901 tgcctgccag cctgcccgtg gagttcacca tcgatgcaaa ggacgccggg gagggcctgc
     6961 tggctgtcca gatcacggat cccgaaggca agccgaagaa gacacacatc caagacaacc
     7021 atgacggcac gtatacagtg gcctacgtgc cagacgtgac aggtcgctac accatcctca
     7081 tcaagtacgg tggtgacgag atccccttct ccccgtaccg cgtgcgtgcc gtgcccaccg
     7141 gggacgccag caagtgcact gtcacagtgt caatcggagg tcacgggcta ggtgctggca
     7201 tcggccccac cattcagatt ggggaggaga cggtgatcac tgtggacact aaggcggcag
     7261 gcaaaggcaa agtgacgtgc accgtgtgca cgcctgatgg ctcagaggtg gatgtggacg
     7321 tggtggagaa tgaggacggc actttcgaca tcttctacac ggccccccag ccgggcaaat
     7381 acgtcatctg tgtgcgcttt ggtggcgagc acgtgcccaa cagccccttc caagtgacgg
     7441 ctctggctgg ggaccagccc tcggtgcagc cccctctacg gtctcagcag ctggccccac
     7501 agtacaccta cgcccagggc ggccagcaga cttgggcccc ggagaggccc ctggtgggtg
     7561 tcaatgggct ggatgtgacc agcctgaggc cctttgacct tgtcatcccc ttcaccatca
     7621 agaagggcga gatcacaggg gaggttcgga tgccctcagg caaggtggcg cagcccacca
     7681 tcactgacaa caaagacggc accgtgaccg tgcggtatgc acccagcgag gctggcctgc
     7741 acgagatgga catccgctat gacaacatgc acatcccagg aagccccttg cagttctatg
     7801 tggattacgt caactgtggc catgtcactg cctatgggcc tggcctcacc catggagtag
     7861 tgaacaagcc tgccaccttc accgtcaaca ccaaggatgc aggagagggg ggcctgtctc
     7921 tggccattga gggcccgtcc aaagcagaaa tcagctgcac tgacaaccag gatgggacat
     7981 gcagcgtgtc ctacctgcct gtgctgccgg gggactacag cattctagtc aagtacaatg
     8041 aacagcacgt cccaggcagc cccttcactg ctcgggtcac aggtgacgac tccatgcgta
     8101 tgtcccacct aaaggtcggc tctgctgccg acatccccat caacatctca gagacggatc
     8161 tcagcctgct gacggccact gtggtcccgc cctcgggccg ggaggagccc tgtttgctga
     8221 agcggctgcg taatggccac gtggggattt cattcgtgcc caaggagacg ggggagcacc
     8281 tggtgcatgt gaagaaaaat ggccagcacg tggccagcag ccccatcccg gtggtgatca
     8341 gccagtcgga aattggggat gccagtcgtg ttcgggtctc tggtcagggc cttcacgaag
     8401 gccacacctt tgagcctgca gagtttatca ttgatacccg cgatgcaggc tatggtgggc
     8461 tcagcctgtc cattgagggc cccagcaagg tggacatcaa cacagaggac ctggaggacg
     8521 ggacgtgcag ggtcacctac tgccccacag agccaggcaa ctacatcatc aacatcaagt
     8581 ttgccgacca gcacgtgcct ggcagcccct tctctgtgaa ggtgacaggc gagggccggg
     8641 tgaaagagag catcacccgc aggcgtcggg ctccttcagt ggccaacgtt ggtagtcatt
     8701 gtgacctcag cctgaaaatc cctgaaatta gcatccagga tatgacagcc caggtgacca
     8761 gcccatcggg caagacccat gaggccgaga tcgtggaagg ggagaaccac acctactgca
     8821 tccgctttgt tcccgctgag atgggcacac acacagtcag cgtcaagtac aagggccagc
     8881 acgtgcctgg gagccccttc cagttcaccg tggggcccct aggggaaggg ggagcccaca
     8941 aggtccgagc tgggggccct ggcctggaga gagctgaagc tggagtgcca gccgaattca
     9001 gtatctggac ccgggaagct ggtgctggag gcctggccat tgctgtcgag ggccccagca
     9061 aggctgagat ctcttttgag gaccgcaagg acggctcctg tggtgtggct tatgtggtcc
     9121 aggagccagg tgactacgaa gtctcagtca agttcaacga ggaacacatt cccgacagcc
     9181 ccttcgtggt gcctgtggct tctccgtctg gcgacgcccg ccgcctcact gtttctagcc
     9241 ttcaggagtc agggctaaag gtcaaccagc cagcctcttt tgcagtcagc ctgaacgggg
     9301 ccaagggggc gatcgatgcc aaggtgcaca gcccctcagg agccctggag gagtgctatg
     9361 tcacagaaat tgaccaagat aagtatgctg tgcgcttcat ccctcgggag aatggcgttt
     9421 acctgattga cgtcaagttc aacggcaccc acatccctgg aagccccttc aagatccgag
     9481 ttggggagcc tgggcatgga ggggacccag gcttggtgtc tgcttacgga gcaggtctgg
     9541 aaggcggtgt cacagggaac ccagctgagt tcgtcgtgaa cacgagcaat gcgggagctg
     9601 gtgccctgtc ggtgaccatt gacggcccct ccaaggtgaa gatggattgc caggagtgcc
     9661 ctgagggcta ccgcgtcacc tataccccca tggcacctgg cagctacctc atctccatca
     9721 agtacggcgg cccctaccac attgggggca gccccttcaa ggccaaagtc acaggccccc
     9781 gtctcgtcag caaccacagc ctccacgaga catcatcagt gtttgtagac tctctgacca
     9841 aggccacctg tgccccccag catggggccc cgggtcctgg gcctgctgac gccagcaagg
     9901 tggtggccaa gggcctgggg ctgagcaagg cctacgtagg ccagaagagc agcttcacag
     9961 tagactgcag caaagcaggc aacaacatgc tgctggtggg ggttcatggc ccaaggaccc
    10021 cctgcgagga gatcctggtg aagcacgtgg gcagccggct ctacagcgtg tcctacctgc
    10081 tcaaggacaa gggggagtac acactggtgg tcaaatgggg gcacgagcac atcccaggca
    10141 gcccccaccg cgttgtggtg ccctaagagc tcaagcttcg aattctgcag tcgacggtac
    10201 cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata ccacatttgt
    10261 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    10321 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    10381 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    10441 caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg
    10501 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
    10561 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
    10621 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
    10681 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
    10741 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
    10801 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
    10861 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
    10921 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    10981 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    11041 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
    11101 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    11161 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    11221 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
    11281 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    11341 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    11401 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
    11461 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
    11521 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
    11581 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
    11641 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
    11701 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
    11761 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
    11821 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
    11881 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
    11941 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
    12001 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
    12061 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
    12121 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
    12181 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
    12241 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
    12301 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
    12361 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
    12421 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
    12481 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
    12541 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
    12601 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
    12661 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
    12721 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    12781 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    12841 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    12901 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    12961 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
    13021 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    13081 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    13141 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    13201 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    13261 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    13321 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    13381 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    13441 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    13501 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
