LOCUS       sequence-106602-       12848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Spectrin-C-term cpstFRET to measure mechanical stress 
            and report multidimensional stress fields.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12848)
  AUTHORS   Meng F, Sachs F
  TITLE     Orientation-based FRET sensor for real-time imaging of cellular 
            forces.
  JOURNAL   J Cell Sci. 2012 Feb 1;125(Pt 3):743-50. doi: 10.1242/jcs.093104.
  PUBMED    22389408
REFERENCE   2  (bases 1 to 12848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2012-02-1"; volume: "125(Pt 3):743-50. doi"; pages: " 
            10.1242/jcs.093104"
COMMENT     Sequence Label: Spectrin-C-cpstFRET
FEATURES             Location/Qualifiers
     source          1..12848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      7353..7362
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7980..8714
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKEFGGGGGVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLS*GLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNSISHN
                     VYITADKQKNGIKANFKILHNIE"
     CDS             join(8949..8951,8952..9464)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIE"
     CDS             9477..9494
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     polyA_signal    9636..9757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9673..9692)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9727..9746
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9764..10219)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9901..9922)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10113..10132
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10246..10350
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10352..10709
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10352..10543
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10375..10395)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10560..10695
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10744..11538
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10744..11538
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10798..10817)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11408..11427
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11726..11745)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11770..11817
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      12146..12734
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12635..12654
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtatgga cccaagtggg gtcaaagtgc tggaaacagc agaggacatc caggagaggc
      661 ggcagcaggt cctagaccga taccaccgct tcaaggaact ctcaaccctt aggcgtcaga
      721 agctggaaga ttcctatcga ttccagttct ttcaaagaga tgctgaagag ctggagaaat
      781 ggatacagga aaaacttcag attgcatctg atgagaatta taaagaccca accaacttgc
      841 agggaaagct tcagaagcat caagcatttg aagctgaagt gcaggccaac tcaggagcca
      901 ttgttaagct ggatgaaact ggaaacctga tgatctcaga agggcatttt gcatctgaaa
      961 ccatacggac ccgtttgatg gagctgcacc gccagtggga attacttttg gagaagatgc
     1021 gagaaaaagg aatcaaactg ctgcaggccc agaagttggt gcagtactta cgagaatgtg
     1081 aggacgtgat ggactggatc aatgacaagg aagcaattgt tacttctgaa gagctgggcc
     1141 aggatctgga gcatgtagag gttttacaga agaaatttga agagtttcaa acagatatgg
     1201 ctgctcatga agaaagagtt aatgaagtga accagtttgc tgccaaactc atacaggagc
     1261 agcaccctga ggaggaactg atcaagacta agcaggatga agtcaatgca gcctggcagc
     1321 ggctgaaggg cctggctctg cagaggcagg ggaagctctt tggggcagca gaagttcagc
     1381 gctttaacag ggatgtggat gagactatca gttggattaa ggaaaaggag cagttaatgg
     1441 cctctgatga ttttggccga gacctggcaa gtgttcaggc tctgcttcgg aagcacgagg
     1501 gtctggagag agatcttgct gctctagaag acaaggtcaa agccctgtgt gctgaggctg
     1561 accgcctgca acagtcccac cctctgagtg caacacagat tcaagtgaag cgagaggaac
     1621 tgattacaaa ctgggagcag atccgcacct tggcggcaga gagacatgca cggctcaatg
     1681 attcatacag gcttcaacgc ttccttgctg acttccgtga cctcaccagc tgggtgactg
     1741 agatgaaagc cctcatcaat gcagatgagc ttgccagtga tgtggctggg gctgaagccc
     1801 tgctagatag acaccaagag cacaagggtg aaattgatgc ccatgaagac agcttcaaat
     1861 ctgcagatga atctggacag gcactgcttg ctgctggtca ttatgcctca gatgaagtga
     1921 gggagaagct gaccgtcctt tccgaggaga gagcggcgct gctggagctg tgggagctgc
     1981 gcaggcagca gtacgagcag tgcatggacc tgcagctctt ctaccgggac actgagcagg
     2041 tggacaactg gatgagcaag caggaggcgt tcctgttgaa tgaagacttg ggagattcct
     2101 tggatagtgt ggaagcgctt cttaagaagc acgaagactt tgagaaatcc cttagtgccc
     2161 aggaggaaaa gattacagca ttagatgaat ttgcaaccaa gctaattcag aacaaccact
     2221 atgcaatgga agatgtggcc actcgccgag atgctctgtt gagccgccgc aatgcccttc
     2281 acgagagagc catgcgtcgc cgggcccagc tagccgattc tttccatctg cagcagtttt
     2341 tccgtgattc tgatgagctc aagagttggg tcaatgagaa gatgaaaact gccacagatg
     2401 aagcttataa agatccatcc aacctacaag gaaaagtaca gaagcatcag gcttttgagg
     2461 ctgagctctc agcaaaccag agccgaattg atgccttgga gaaagctggc caaaagctga
     2521 ttgatgtcaa ccactatgcc aaggatgaag tggcagctcg tatgaatgag gtgatcagtt
     2581 tgtggaagaa actgctagag gccactgaac tgaaaggaat aaagcttcgt gaagccaacc
     2641 agcaacagca atttaatcgc aatgttgagg atattgaatt gtggctatat gaagtagaag
     2701 gtcacttggc ttcggatgat tacggcaaag atcttaccaa tgtgcagaac ctccagaaga
     2761 aacatgccct gctagaggca gatgtggctg ctcaccagga ccgaattgat ggcatcacca
     2821 ttcaggcccg ccagttccaa gatgctggcc attttgatgc agaaaacatc aagaagaaac
     2881 aggaagccct cgtggctcgc tatgaggcac tcaaggagcc catggttgcc cggaagcaga
     2941 agctggccga ttctctgcgg ttgcagcagc tcttccggga tgttgaggat gaggagacgt
     3001 ggattcgaga gaaagagccc attgccgcat ctaccaacag aggtaaggat ttaattgggg
     3061 tccagaatct gctaaagaaa catcaagcct tacaagcaga aattgctgga catgaaccac
     3121 gcatcaaagc agttacacag aaggggaatg ccatggtgga ggaaggccat tttgctgcag
     3181 aggatgtgaa ggccaagctt cacgagctga accaaaagtg ggaggcactg aaagccaaag
     3241 cttcccagcg tcggcaggac ctggaggact ctctgcaggc ccagcagtac tttgctgatg
     3301 ctaacgaggc tgaatcctgg atgcgggaga aggaacccat tgtgggcagc actgactatg
     3361 gcaaggacga agactctgct gaggctctac tgaagaaaca cgaagctttg atgtcagatc
     3421 tcagtgccta cggcagcagc atccaggctt tgcgagaaca agcacagtcc tgccggcaac
     3481 aagtggcccc cacggatgat gagactggga aggagctggt cttggctctc tacgactatc
     3541 aggagaagag tccccgagag gtcaccatga agaagggaga tatccttacc ttactcaaca
     3601 gcaccaacaa ggattggtgg aaagtggaag tgaacgatcg tcagggtttt gtgccggctg
     3661 cgtacgtgaa gaaattggac cccgcccagt cagcctcccg ggagaatctc ctggaggagc
     3721 aaggcagcat agcactgcgg caggagcaga ttgacaatca atatcattct ctgctggaac
     3781 tgggtgagaa gcgtaaaggc atgttggaga agagttgcaa gaagtttatg ttgttccgtg
     3841 aagcgaatga actacagcaa tggatcaatg agaaggaagc cgctctgaca agtgaggagg
     3901 tcggagcaga cttggagcag gttgaggtgc tccagaagaa gtttgatgac ttccagaagg
     3961 acctgaaggc caatgagtca cggttgaagg acattaacaa ggtagctgaa gacctggagt
     4021 ctgaaggtct tatggcagag gaggtgcagg ctgtgcaaca acaggaagtg tatggcatga
     4081 tgcccaggga tgaaactgat tccaagacag cctccccgtg gaagtctgct cgtctgatgg
     4141 ttcacaccgt ggccaccttt aattccatca aggagctgaa tgagcgctgg cggtccctac
     4201 agcagctggc cgaggaacgg agccagctct tgggcagcgc ccatgaagta cagaggttcc
     4261 acagagatgc tgatgaaacc aaagaatgga ttgaagagaa gaatcaagct ctaaacacag
     4321 acaattatgg acatgatctc gccagtgtcc aggccctgca acgcaagcat gagggcttcg
     4381 agagggacct tgcggctctc ggtgacaagg taaactccct tggtgaaaca gcagagcgcc
     4441 tgatccagtc ccatcccgag tcagcagaag acctgcagga aaagtgcaca gagttaaacc
     4501 aggcctggag cagcctgggg aaacgtgcag atcagcgcaa ggcaaagttg ggtgactccc
     4561 acgacctgca gcgcttcctt agcgatttcc gggacctcat gtcttggatc aatggaatac
     4621 gggggttggt gtcctcagat gagctagcca aggatgtcac cggagctgag gcattgctgg
     4681 agcgacacca ggaacaccgg acagaaatcg atgccagggc tggcactttc caggcatttg
     4741 agcagtttgg acagcagctg ttggctcacg gacactatgc cagccctgag atcaagcaga
     4801 aacttgatat tcttgaccag gagcgtgcag acctggagaa ggcctgggtt cagcgcagga
     4861 tgatgctgga tcagtgcctt gaactgcagc tgttccatcg ggactgtgag caagctgaga
     4921 actggatggc tgcccgggag gccttcttga ataccgaaga caaaggagac tcactggaca
     4981 gcgtagaggc tctgatcaaa aaacatgaag actttgacaa agcgattaac gtccaggaag
     5041 agaagattgc tgctctgcag gcctttgccg accagctcat cgctgccggc cattatgcca
     5101 agggagacat ttctagccgg cgcaatgagg tcttggacag gtggcgacgt ctgaaagccc
     5161 agatgattga gaaaaggtca aagctaggag aatctcaaac cctccaacag ttcagccggg
     5221 atgtggatga gattgaggct tggatcagtg aaaaattgca aacagcgagt gatgagtcgt
     5281 acaaggatcc caccaacatc cagagcaagc accagaagca ccaggctttt gaagcagagc
     5341 tgcatgccaa cgctgaccgg atccgtgggg ttatcgacat gggcaactcc ctcattgaac
     5401 gtggagcctg tgccggcagt gaggatgctg tcaaggcccg cctggctgcc ttagctgacc
     5461 agtggcagtt cttggtgcaa aagtcagcgg aaaagagcca gaaactgaaa gaagccaaca
     5521 agcagcagaa cttcaacaca gggatcaagg actttgactt ctggctgtct gaggtggagg
     5581 ccctgctggc atccgaagat tatggcaaag acctggcttc tgtgaacaac ctgctgaaaa
     5641 agcatcaact gctggaagca gatatatctg cccatgagga tcgcctgaag gacctgaaca
     5701 gccaggcaga cagcctgatg accagcagtg ccttcgacac ctcccaagta aaggacaaga
     5761 gggacaccat caacgggcgc ttccagaaga tcaagagcat ggcggcctcc cggcgagcca
     5821 agctgaatga atcccatcgc ctgcaccagt tcttccggga catggatgac gaggagtcct
     5881 ggatcaagga gaagaagctg ctggtgggct cagaggacta cggccgggac ctaactggcg
     5941 tgcagaacct gaggaagaag cacaagcggc tggaagcaga actggctgcg catgagccgg
     6001 ctattcaggg tgtcctggac actggcaaga agctgtccga tgacaacacc atcgggaaag
     6061 aggagatcca gcagcggctg gcgcagtttg tggagcactg gaaagagctg aagcagctgg
     6121 cagctgcccg gggtcagcgg ctggaagagt ccttggaata tcagcagttt gtagccaatg
     6181 tggaagagga agaagcctgg atcaatgaga aaatgaccct ggtggccagc gaagattatg
     6241 gcgacactct tgccgccatc cagggcttac tgaagaaaca tgaagctttt gagacagact
     6301 tcaccgtcca caaggatcgc gtgaatgatg tctgcaccaa tggacaagac ctcattaaga
     6361 agaacaatca ccatgaggag aacatctctt caaagatgaa gggcctgaac gggaaagtgt
     6421 cagacctgga gaaagctgca gcccagagaa aggcgaagct ggatgagaac tcggccttcc
     6481 ttcagttcaa ctggaaggcg gacgtggtgg agtcctggat cggtgaaaag gagaacagct
     6541 tgaagacaga tgattatggc cgagacctgt cttctgtgca gacgctcctc accaaacagg
     6601 aaacttttga cgctgggctg caggccttcc agcaggaagg cattgccaac atcactgccc
     6661 tcaaagatca gcttctcgcc gccaaacacg ttcagtccaa ggccatcgag gcccggcacg
     6721 cctccctcat gaagaggtgg agccagcttc tggccaactc agccgcccgc aagaagaagc
     6781 ttctggaggc tcagagtcac ttccgcaagg tggaggacct cttcctgacc ttcgccaaaa
     6841 aggcttctgc cttcaacagc tggtttgaaa atgcagagga ggacttaaca gaccccgtgc
     6901 gctgcaactc cttggaagaa atcaaagctt tgcgcgaggc ccacgacgcc ttccgctcct
     6961 ccctcagctc tgcccaggct gacttcaacc agctggccga gctggaccgc cagatcaaga
     7021 gcttccgcgt agcctccaac ccctacacct ggtttaccat ggaggccctg gaggagacct
     7081 ggaggaacct acagaaaatc atcaaggaga gggagctgga gctgcagaag gaacagcggc
     7141 ggcaggagga gaacgacaag ctgcgccagg agtttgccca gcacgccaac gccttccacc
     7201 agtggatcca agagaccagg acatacctcc tcgatgggtc ctgtatggtg gaagagtcgg
     7261 ggaccctcga atcccagctt gaagctacca aacgcaagca ccaggaaatc cgagccatga
     7321 gaagtcagct caaaaagatc gaggacctgg gggccgccat ggaggaggcc ctcatcctgg
     7381 acaacaagta cacggagcac agcaccgtgg gcctcgccca gcagtgggac cagctggacc
     7441 agctgggcat gcgcatgcag cacaacctgg agcagcagat ccaggccagg aacacaacag
     7501 gtgtgactga ggaggccctc aaagaattca gcatgatgtt taaacacttt gacaaggaca
     7561 agtctggcag gctgaaccat caggagttca aatcttgcct gcgctccctg ggctatgacc
     7621 tgcccatggt ggaggaaggg gaacctgacc ctgagttcga ggcaatcctg gacacggtgg
     7681 atccgaacag agatggccat gtctccttgc aagaatacat ggctttcatg atcagccgcg
     7741 aaactgagaa cgtcaagtcc agcgaggaga ttgagagcgc cttccgggcc ctcagctcag
     7801 agggaaagcc ttacgtgacc aaggaggagc tctaccagaa cctgacccgg gaacaagccg
     7861 actactgcgt ctcccacatg aagccctacg tggacggcaa gggccgcgag ctccccaccg
     7921 cgttcgacta cgtggagttc acccgctcgc ttttcgtgaa cggtaccgta ccaggatcca
     7981 tgggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     8041 tgctgctgcc cgacaaccac tacctgagct accagtccaa actgagcaaa gaccccaacg
     8101 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     8161 tggacgagct gtacaaggaa ttcggcggcg gtggcggggt gagcaagggc gaggagctgt
     8221 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     8281 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct
     8341 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg agctagggcc
     8401 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     8461 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     8521 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     8581 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tccatcagcc
     8641 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
     8701 tccacaacat cgaggacggc ggcagatcta tgggcagcgt gcagctcgcc gaccactacc
     8761 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     8821 cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     8881 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggaa ttcggcggcg
     8941 gtggcggggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     9001 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     9061 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     9121 ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cgcccgctac cccgaccaca
     9181 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     9241 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     9301 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     9361 ggcacaagct ggagtacaac gccatcagcg acaacgtcta tatcaccgcc gacaagcaga
     9421 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacgag ctcgctctgg
     9481 tgccacgcgg tagttccggt ggcagcggcc gcaccggatc tagataactg atcataatca
     9541 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     9601 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     9661 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     9721 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat
     9781 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     9841 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     9901 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     9961 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
    10021 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
    10081 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
    10141 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
    10201 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
    10261 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
    10321 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg
    10381 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    10441 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
    10501 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
    10561 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
    10621 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
    10681 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt
    10741 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
    10801 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
    10861 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
    10921 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
    10981 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
    11041 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
    11101 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
    11161 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
    11221 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
    11281 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
    11341 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
    11401 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
    11461 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
    11521 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
    11581 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
    11641 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
    11701 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
    11761 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata
    11821 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
    11881 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
    11941 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata
    12001 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    12061 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    12121 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    12181 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    12241 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
    12301 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    12361 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    12421 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    12481 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    12541 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    12601 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    12661 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    12721 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    12781 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    12841 ccatgcat
//
