LOCUS       sequence-106608-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RPL16b HA-TEV-AVI pop-in/pop-out plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Jan CH, Williams CC, Weissman JS
  TITLE     Principles of ER cotranslational translocation revealed by 
            proximity-specific ribosome profiling.
  JOURNAL   Science. 2014 Nov 7;346(6210):1257521. doi: 10.1126/science.1257521.
            Epub 2014 Nov 6.
  PUBMED    25378630
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2014-11-7"; volume: "346(6210):1257521. doi"; pages: " 
            10.1126/science.1257521. Epub 2014 Nov 6"
COMMENT     Sequence Label: pJW1506
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(334..378)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(382..402)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(412..438)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(849..865)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(850..866)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgc
       61 atattgttat tctttcacgc tttgttttga tatggtgaaa attcgaagtc tctagaatgt
      121 cctttaggta cctttcttta agatgtggtt catattcaaa aaatctgatc aattagaatt
      181 tataccagtg ttttaatatg tttatttatc tgttattgat attatttaaa atgtgaaaca
      241 aaataaataa aaataactaa aaatgtaggg tggtttgttg ttttgcaaaa aataaaagat
      301 aagattgatc acaaattcat ttcatgaaat ttactcatgc cattcaattt tttgagcttc
      361 aaaaatatca ttcaaacctg atccttgaaa atacaaattt tctgaacctc cagcgtagtc
      421 tgggacgtcg tatgggtaag aaccgtaacc gaaagaagcc aattgcttag caacatcgga
      481 ttcagaagca gcagcagaag cagaggagac ctttttggtg aaagctctct tcttggcgta
      541 atattcggcg gatctgacct ttctcttgtc ttctaattta gcaacaacat cttcgtattt
      601 ccaaccaaca gaagtagaca acttacccaa agtggtgtac tttctacctg gtttcaatct
      661 caaaactctc aaagcttgag gaacgacaac tctcttcttt ttgtcgtatg gaggtggaat
      721 accttcgaag atctttaaac gttccaaggc agccttacca cgggcggtct tgtgggaaac
      781 cataccacga atagccttgt aaagactagt ggatcccccg ggctgcagga attcgatatc
      841 aagcttatcg ataccgtcga cctcgaggg
//
