LOCUS       sequence-106624-        6388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter plasmid used to study transcriptional control of
            murine Tet1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Sohni A, Bartoccetti M, Khoueiry R, Spans L, Vande Velde J, De 
            Troyer L, Pulakanti K, Claessens F, Rao S, Koh KP
  TITLE     Dynamic switching of active promoter and enhancer domains regulates 
            Tet1 and Tet2 expression during cell state transitions between 
            pluripotency and differentiation.
  JOURNAL   Mol Cell Biol. 2015 Mar 15;35(6):1026-42. doi: 10.1128/MCB.01172-14.
            Epub 2015 Jan 12.
  PUBMED    25582196
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01172-14"; journalName: "Mol Cell Biol"; date: 
            "2015-03-15- 15"; volume: "35"; issue: "6"; pages: "1026-42"
COMMENT     Sequence Label: pGL3-SV40P-Tet1 enhancer E
FEATURES             Location/Qualifiers
     source          1..6388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..233
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      84..219
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      263..272
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             269..1921
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(335..353)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    1962..2083
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1973..1992)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2027..2046
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3720..3737)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3891..4479)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3971..3990)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4650..5441,5442..5510))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5273..5292
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5511..5615)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5642..6097
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5729..5748)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5939..5960
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    6228..6276
                     /note="synthetic polyadenylation signal"
     misc_feature    6290..6381
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6330..6349
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctaggatct gcgatctgca tctcaattag tcagcaacca
       61 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
      121 cgccccatcg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
      181 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
      241 gcattccggt actgttggta aagccaccat ggaagacgcc aaaaacataa agaaaggccc
      301 ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa
      361 gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat
      421 cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg
      481 gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc
      541 ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga
      601 acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa
      661 ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat
      721 catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca
      781 tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac
      841 aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc
      901 tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat
      961 cattccggat actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac
     1021 tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga
     1081 gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct
     1141 attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga
     1201 aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt
     1261 ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat
     1321 tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc
     1381 gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg
     1441 tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt
     1501 gattgacaag gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca
     1561 cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc
     1621 cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg
     1681 tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa
     1741 gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa
     1801 gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga
     1861 cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta
     1921 attctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca ttgatgagtt
     1981 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     2041 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     2101 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     2161 ctacaaatgt ggtaaaatcg ataaggatcc gtcgacggct gtgagtgcag atcttcattg
     2221 atcatatagg gcattgccct ggtcttcata tccccaatta agcattttga attggggtct
     2281 ttttaaaaat aaacatttgg aggaatagga tttatttagg gtctttagta ggctggacta
     2341 aatgactgta gaactcaaca ttttaatgag ttaactattg ctctcagatt ccctgcttcc
     2401 gctgaagggg tgggtcagag gttggagaca ccctcctcac cctttgtctc ttccttatct
     2461 accttcttcc cctcccccac cttctggaca catccttcct gcctccttca tttcttttcc
     2521 cccttgtcct taagaaaaaa aaaatttttt tttaaacctg tatgtctttt gattctcctt
     2581 cccctacctg acctcagttc tcttctgttg tctttaactc cttacattct tgtgaattct
     2641 tggctttgct cagtcgtttg gatttctatc ttttcacctt ataaaatgta taagatactg
     2701 ttccttagcc tcttcctgtt cttccttaaa ccgcctggct tttggaagag acttttaacc
     2761 tctttttttc ccctgttctc ttccattttg gttctctttg cgtcccccac ccgaccctgc
     2821 ccccgtgggg tgtccggggg tgttttacat atggaaggag gcagctttca gctgcatttc
     2881 cctgagcagc ctctccgagc gccccaggct ttcacacttc ctgagatagc ctcccccccc
     2941 ccccccttca atgtagaatc tagttgtggg gaaatccagc ccagtgtcaa cttttttcag
     3001 aaaatgcttt taataagtct tatattagtc ttagtgtttg gtttggtttg gttggtccag
     3061 gggaaaagcc tttatggtct ctgggcctga aatcagtttg cgtgtagctt taggcatgca
     3121 aatgacactt tagcaccggc ggcagctgga aggattgcag tgttatttca aattgatgtt
     3181 tgggctcaat ggttacttaa gaaagtgcag cagccattgc ctgctcaggg gacttccaga
     3241 ggtaagcaag agaagagatt ttcgagggag aggaaattcc ccgatggtgc catcaatact
     3301 gtaattcacg gcctactggt attgttagga agctagcctt tcattttttt taggtaagga
     3361 gaataaatac ttttattttt ttttttattt tgtttttgaa gacagggttt ctctgtgtag
     3421 ctctggctac cctggaactt gctctgtaga ccaggctggc cttgaactca tgaggtccac
     3481 ctgcctctgc ctcaccccca ctaccctcag tgtcaactct gactagtttt attttcattt
     3541 tcctccctgg acaataagga ctcaatagga tcctgtcagg tcgaccgatg cccttgagag
     3601 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     3661 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc
     3721 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     3781 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3841 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3901 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3961 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4021 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4081 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4141 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4201 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4261 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4321 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4381 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4441 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4501 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4561 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4621 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4681 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4741 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4801 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4861 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4921 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4981 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5041 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     5101 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5161 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5221 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     5281 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5341 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5401 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5461 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5521 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5581 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5641 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5701 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5761 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5821 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     5881 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5941 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6001 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6061 aacgcgaatt ttaacaaaat attaacgttt acaatttccc attcgccatt caggctgcgc
     6121 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc caagctacca
     6181 tgataagtaa gtaatattaa ggtacgggag gtacttggag cggccgcaat aaaatatctt
     6241 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc
     6301 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg
     6361 caggtgccag aacatttctc tatcgata
//
