LOCUS       sequence-106627-        9838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter plasmid used to study transcriptional control of
            murine Tet1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9838)
  AUTHORS   Sohni A, Bartoccetti M, Khoueiry R, Spans L, Vande Velde J, De 
            Troyer L, Pulakanti K, Claessens F, Rao S, Koh KP
  TITLE     Dynamic switching of active promoter and enhancer domains regulates 
            Tet1 and Tet2 expression during cell state transitions between 
            pluripotency and differentiation.
  JOURNAL   Mol Cell Biol. 2015 Mar 15;35(6):1026-42. doi: 10.1128/MCB.01172-14.
            Epub 2015 Jan 12.
  PUBMED    25582196
REFERENCE   2  (bases 1 to 9838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01172-14"; journalName: "Mol Cell Biol"; date: 
            "2015-03-15- 15"; volume: "35"; issue: "6"; pages: "1026-42"
COMMENT     Sequence Label: pGL3-Tet1 promoter A
FEATURES             Location/Qualifiers
     source          1..9838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      5102..5111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5108..6760
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5174..5192)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    6801..6922
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6812..6831)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6866..6885
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7170..7187)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7341..7929)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7421..7440)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8100..8891,8892..8960))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8723..8742
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8961..9065)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9092..9547
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9179..9198)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9389..9410
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    9678..9726
                     /note="synthetic polyadenylation signal"
     misc_feature    9740..9831
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     9780..9799
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt ctttggaaga gcagtcagtg ctcttaaccg ctgagccatc
       61 tctccagccc cccccccccc cccccccccc ccccgtcttt tgacttccaa atgtatatac
      121 attgtggcac ccaacactct ccacttaata cataattaat ggctggtcag tggtggctca
      181 cctctttaat cccagcactt gggaggcaga ggcaggtgga tttctgagtt caaggccaac
      241 ctggtctaca gagtgagttc caggacagct aaggctacac ggagaaaccc tgtctcgaaa
      301 aatcaaaaaa ccaaaaccaa acaaacaaaa aaaactaaaa aaacataatg taatataaag
      361 gtctatgtac ttttgcccag tgagccattg cactgatctc aaattctgcc ttgggaagat
      421 gtggaggctc tcgtctgtaa tctcaggaat aggaagttat gtctataagg tttctgcagg
      481 ttcaaaacat cagcactaga ttgtaagttt caggaaagcc taggatacaa aatcaaaatc
      541 agagctggag agatagctca gcggttaaga gcactgactg ctcttccaga ggtcctgtcc
      601 cagtaaccat gtggtgactc atgaccatct gtaatgggaa ccgatgcctc cttcttgtgt
      661 gtctaaagac tgcaatccta tactcatata aacaaagaat aaaatcgcca ggcatggtgg
      721 cacccgcctc taatcccagc acttgggagg tagaggcagg tggatttctg agttccaagc
      781 cagcctgatc caaagagtga gtcaaaaaaa gtcaactacc cagggctatt gagatggaac
      841 agacagactt gtggccaagt ctgatgacca tccatccctg agacccatgc agtggagttg
      901 gagagctagc actcacaagt tgtcttctga cctccaccca ggcacacttc aagtctccaa
      961 acacatgaag cagtgttgaa catttttccc cctaaacaag atgatttatg attggggaag
     1021 tggctaagtg gcaggttact tgcttaaggt gccagcaata ctgcaaaaga gaaaccagaa
     1081 agatccggta aaatgtaaca atgaaacgat aatttgaagt tacttaaaag tatcaaattg
     1141 tagcagagct cacgggatca atgtaagcaa gaccaacctg gtgtgtatag ggaattcagg
     1201 ccagttacca ctacaaaggg agactgtctc aaaaactaaa ataaaaatac aaaagcccca
     1261 aacagccggg cagtggtggc acacaccttt aattcctgca cttgagaggt agaggcaggc
     1321 agatttttga gttcaagggc agcctggtct gcagaatgag ttccaggaca ggcagggcta
     1381 cacagagaaa ctctgtcttg aaacaaaaca aaacaaaata aaacaaaata aatgaagccc
     1441 caaacaacaa caccacccaa aaaaaaaaaa atcttatgga ttgggctagg gatagagctc
     1501 atataccaaa taaagcagac aaataaggtc tgtagtaaat tttgagagta aacatggcat
     1561 atgtgccttg aaaagtaaga tctgagtttg attcctacta tacacatggt gatggctagg
     1621 tacggtatta aagccttgta agaacagtaa tacactgctg ggcctggtgt tgcatgcctt
     1681 ttatcccagc acttgggagg cagaggcagg tggatttctg aggccagcct ggtctgcaga
     1741 gtgagttcca agacagccag aactatacag agaaaaaccc tgtctcgaaa aacaaagaac
     1801 aataattaca catggcagga aaatatcact tagttctgta atctgagctt ctctggagtc
     1861 ttttttttgt tttgtttttg tttttgaaga cagggtttct ctgtttagct ctggctgtcc
     1921 tgtagaccag gttgtcctcg aactcagaaa tctgcctgcc tctgctgccc aagtgctggg
     1981 attaaaggtg tgtgccacca tgcctggctt tttcttcttt ctttctttct ttccttcctt
     2041 ccttccttcc ttccttcttt ctttctttct ttctttcttt ctttctttct ttctttcttt
     2101 ctttctttct ttctttcttt ctcatttgtt atgtaagtgc attgtagttg tcttcagaca
     2161 ggccagaaga cagcatcaga tctcattatg gtggttgtga gccatgttgg gatttgaact
     2221 caggacctgg gaaataacag tcagtgctct tagccactga gccatttcgc ctgcccaaag
     2281 acctgagttt ctttctttct tttcttttat aattaattaa ttaattaatt aattatatgt
     2341 aagtacacca tagctgtcct catacactcc agaagaggga gtcagatctc atttcagatg
     2401 gttgtgagcc accatgtggt tgctgggatt tgaactcagg actttaggaa gagcagtggg
     2461 cactcttaac cgctgagcca tctcaccagc cccaagacct gagtttctta aaggggtcat
     2521 ggaacagtcc ctggcagaca agactgatat gctacacagt tgccagtggc aaattgcttg
     2581 atgggttgag gaaaagtcca caaacctgga cacagaacta actattcact gctgaggtgg
     2641 tgtgagcaga gaaataaaaa tatgttgttt gtttccttca tatgactaga aaacatcaga
     2701 aaaatcccag ctcctgataa gctattgtaa atggagtagt aactgccttg catgctatta
     2761 tctctggaga cattgccttt agtaccagtc ctgcttcgtc cttttttgag tccttgcatt
     2821 aagccttgcc ttcctgtgaa tttttgtatt ctcctgttct cttaaatttg tgcttatgcc
     2881 aaattgtggg agtgagttct cgacaccctg atgtgtggat ttcaaggatc aaactcagat
     2941 ctgtaggctt ggccagaggt actgataatc tgtcccattt caaaggcccg actttatctt
     3001 ttttttcttt tttaaaaatt aaatttatta gaggcaggtg gatttctgag ttcgaggaca
     3061 gctttggtct acaaagcaag tttcaggaca gccagatata caaagaaacc ctgtctcaaa
     3121 aaaaaaaaaa aaaaaaaaaa aaaaaaccaa aaattattta cattccaaat gttgcctccc
     3181 aaaggcccaa cttgaaaaga attttttttg ttgtttgttt tttgagaaaa gaaaatttta
     3241 agtccggcat ggtggcagaa gcctttaatc ccagcactcg ggaggcagag acaggtggac
     3301 aggggtgttg gggctggaga gatggctcag tggttaagaa catttactgc tcttccaaag
     3361 gtcctgagtt caattcccag caaccacata gtgtggctca caacttttgt aacaggatca
     3421 gatgccctct tctggtgtgt ctgaagacag ctctagtgta cttgtcatat atataaaata
     3481 aataaataaa atcttaaaaa actattacag ggtctcactc tattaaggct ggctgggaaa
     3541 agagatgttt gcctggtttt gctagagtgt tttgtcttat ttgtctaaaa acttatctgt
     3601 caatatttct cgcactttat aggtatggga aggagaatca gttcaaggtc aaaatctaat
     3661 agagaaaggg aatgaaagaa aaataaagaa aaaaagagag agccaggttt gcttttttct
     3721 attgcaaaga ttaaattgag gcctcagtct tcacctctga gcagaactca gctagaagtg
     3781 catttctgac ctccaatcag gttactttat aatcccagca ctggaaaggc tgtggctgga
     3841 agattcctgc ctttgagggc agcctgggct acacagagac ttttaaaagc taaacaaaca
     3901 aacccgttat agctggtacc tagttaagaa tacatttaaa ggatttattt agggtgtaag
     3961 cttgacagtt tgggactatt tctccgaaca atctagggac atttatttgt tttattttgt
     4021 tttgtttttt tgagacagga tttctctgta tagccctggc tgtcctagaa ctcactttgt
     4081 aaaccagact ggcctataat tcagaaatcc acttgcctct gccccccaag tcctgggatt
     4141 aaaggcatct gctaggctat cgagggacat ttatatacca cagttcaggt atggaattgg
     4201 gggagtggag tgtaaagcca caagccagtt ttctcccttc cacctgaagc agtagatttc
     4261 cttgtctaag ggcagacttc tggaagaaga gaaagctatc ttaccttggg atatgcctag
     4321 ttaaagtcaa gcctgaacct catggcagga atttctctct tactgtattt ccatttccta
     4381 taaagtgatt atgatcacta atgagtccgc agtcaatggc taatcaaaat aaagatgtag
     4441 ggaaaaaatc aaagctaatt tgatttctct ttggtcttga agttggtttg acacctctag
     4501 gatgtcacct ggatgacccg actcgcttct ggaaacattc gttttcgaga tacctttact
     4561 cccatctgat tagagatact ctgactccca tcactgaaag agatggtgac ctgtagattt
     4621 caacactcct ttgacctaag aaaaaagggg agggagcgcg ggcggaattt caggaaagcc
     4681 taggaaggag tggacaggag aagggtcgag aggagtccgg ctccgatttg ggaaaatcca
     4741 gctcgcattt tgcctctcgc tcagcggtac agggtccagc aaaggctgtg ccccagctac
     4801 actcctagag gtctgtactg gggagacacc gctgctctgg gggggggcga gggctgacct
     4861 ggctgggagt acccgcgcac tccgctcgtg gaacactgca ctctgcaact ggtttgtaca
     4921 atcccggggt ctgtattcgc ccctggacgt cctctccctc cctggtcatg tacctctacc
     4981 tgcctcttct acgggaacat tcgcggagca gctccccaac ccgggtgctc gggccctgac
     5041 cgtgccggat gctcgagatc tgcgatctaa gtaagcttgg cattccggta ctgttggtaa
     5101 agccaccatg gaagacgcca aaaacataaa gaaaggcccg gcgccattct atccgctgga
     5161 agatggaacc gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg
     5221 aacaattgct tttacagatg cacatatcga ggtggacatc acttacgctg agtacttcga
     5281 aatgtccgtt cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat
     5341 cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat
     5401 cggagttgca gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat
     5461 gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa
     5521 cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc atggattcta aaacggatta
     5581 ccagggattt cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga
     5641 atacgatttt gtgccagagt ccttcgatag ggacaagaca attgcactga tcatgaactc
     5701 ctctggatct actggtctgc ctaaaggtgt cgctctgcct catagaactg cctgcgtgag
     5761 attctcgcat gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt
     5821 aagtgttgtt ccattccatc acggttttgg aatgtttact acactcggat atttgatatg
     5881 tggatttcga gtcgtcttaa tgtatagatt tgaagaagag ctgtttctga ggagccttca
     5941 ggattacaag attcaaagtg cgctgctggt gccaacccta ttctccttct tcgccaaaag
     6001 cactctgatt gacaaatacg atttatctaa tttacacgaa attgcttctg gtggcgctcc
     6061 cctctctaag gaagtcgggg aagcggttgc caagaggttc catctgccag gtatcaggca
     6121 aggatatggg ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa
     6181 accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac
     6241 cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt gtgagaggtc ctatgattat
     6301 gtccggttat gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct
     6361 acattctgga gacatagctt actgggacga agacgaacac ttcttcatcg ttgaccgcct
     6421 gaagtctctg attaagtaca aaggctatca ggtggctccc gctgaattgg aatccatctt
     6481 gctccaacac cccaacatct tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg
     6541 tgaacttccc gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat
     6601 cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt
     6661 tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat
     6721 cctcataaag gccaagaagg gcggaaagat cgccgtgtaa ttctagagtc ggggcggccg
     6781 gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     6841 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     6901 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     6961 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga
     7021 taaggatccg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     7081 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     7141 gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7201 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7261 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7321 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7381 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7441 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7501 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     7561 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7621 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7681 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7741 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     7801 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7861 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7921 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7981 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8041 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8101 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8161 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8221 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8281 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8341 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8401 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8461 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8521 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8581 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8641 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8701 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8761 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8821 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8881 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8941 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9001 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9061 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     9121 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     9181 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     9241 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     9301 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     9361 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     9421 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     9481 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     9541 acaatttccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     9601 tcttcgctat tacgccagcc caagctacca tgataagtaa gtaatattaa ggtacgggag
     9661 gtacttggag cggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt
     9721 tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac
     9781 tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tatcgata
//
