LOCUS       sequence-106630-       11003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter plasmid used to study transcriptional control of
            murine Tet1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Sohni A, Bartoccetti M, Khoueiry R, Spans L, Vande Velde J, De 
            Troyer L, Pulakanti K, Claessens F, Rao S, Koh KP
  TITLE     Dynamic switching of active promoter and enhancer domains regulates 
            Tet1 and Tet2 expression during cell state transitions between 
            pluripotency and differentiation.
  JOURNAL   Mol Cell Biol. 2015 Mar 15;35(6):1026-42. doi: 10.1128/MCB.01172-14.
            Epub 2015 Jan 12.
  PUBMED    25582196
REFERENCE   2  (bases 1 to 11003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01172-14"; journalName: "Mol Cell Biol"; date: 
            "2015-03-15- 15"; volume: "35"; issue: "6"; pages: "1026-42"
COMMENT     Sequence Label: pGL3-Tet1 promoter B
FEATURES             Location/Qualifiers
     source          1..11003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      6267..6276
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6273..7925
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(6339..6357)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    7966..8087
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7977..7996)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8031..8050
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8335..8352)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8506..9094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8586..8605)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9265..10056,10057..10125))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9888..9907
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10126..10230)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10257..10712
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10344..10363)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10554..10575
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    10843..10891
                     /note="synthetic polyadenylation signal"
     misc_feature    10905..10996
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     10945..10964
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt tggatgctct tgactgctga gtcacctctc catgtccctt
       61 agaaaggtct tttttctttt ctttttttct tttttttctt ttttgtgaga tagggttttt
      121 ctgtagccct ggctgtcctg gaattcactc tatagggctt tgaactcaga aattcgccta
      181 cctctgcctt ccaagtgctg ggattaaagg cgtgtgccac cattgcccag gaaaggtttt
      241 tctaagaaac aactttagcc atttgaggtg gtccacgcct ttaatcccag cacttgggag
      301 gcagaggcag gcagacttct cagtttgagg ccagcctggt ctacagagtg agttccagga
      361 cagtcaggga aacacaaaga aaccttgtct cgaaaaaatt aaaacaaaac aaaacaaaaa
      421 aaacctttaa acttctttta gaaaacaggc ctttgtggtt taagcaattt gaaacttgga
      481 agcctgattg cctaagggac ttgtgtctat gtgtccgtcc ttggcaggtg aatccactag
      541 aggttgtgga ttcctcccct ccccgactcc tctcctagct ctgcagatca aggacagtag
      601 ccagccaagg gtgtggtgac tgtggaggtc acctgagagg aaacaaccct ccccctgaag
      661 ggtaaaaaga gatattgact aaattatctt tggaagtcta ggctgctcac ctacattaaa
      721 ggactatgaa tcaaaaaaga acccctcccc cagcttctgt gcttccagct gctttgccct
      781 gtgcttctgc cctgtgtctg agcctggctg ggcagcacct tttgtcttta ctcatgactc
      841 cctttatgcc tggaccttgg gatggtaata gacatccttc tgtactgggg actacattta
      901 tttatttttc ttggaatttg aatcactttt ttttaaaatt aattaattaa tttatttatt
      961 tatttaatgt aagtacactg tagctgtctt cagacgcacc agaagagggc atcagatctc
     1021 attacgggtg gttgtgagcc accatgtggt tgctgggatt tgaactctgg accttcggaa
     1081 gagcagtcgg gtgctcttac ccactgagcc atctcaccag cccattgaat cactttctat
     1141 ttgtattgag cacagaaaat ttgtattcct gtagggcaat gatggcatcc gcctttcatc
     1201 tctggttgca tagttcaggt tggatttctg agttccatgc cagcctgctc tacagaatga
     1261 gttccagggc atccagagct agagggagat ttctgagaac aaagttaata gtcttgtatc
     1321 ctgttcttcc ttaacccatg ttgtccctgg aatgtgattt tcctgcccct ccctggcctg
     1381 gtaaacagga gtgggtttac tgtaattatt cctcatgtta tggttattat tcaatgtttt
     1441 tgtgcacaat tgtccttgtg actttacata gcctgaactc atatgacctt gactaaccct
     1501 cagaataatc ctcagaatat aaattgtata atgccctgaa taaagatggt tattgcttga
     1561 gactttacac ctcagtttta ggctccactc tcccgcgggt tcaagccact gactagaaca
     1621 ctaacagtag accaggctgg ccttgaactc agagatccaa gggtctctcc ctcccaagtg
     1681 tcagaattaa aggcaagtac accaccccag gtcgagagag cttttaaaaa ttaagtatgt
     1741 atatttgaat gggtgtgatt gacagcattg gtaaggcggg gcctttgtcc ttgagaaggt
     1801 ctgttgtggc tctggctgac ctgaaagctc cctgtggagc caagggtgac cttgagcttc
     1861 cagtcctctt tgtaggtgag tctgcagcac tttagcaggt tacagggtta cctagttaat
     1921 taacttgata tagcgtctcc tgtagaccag gctgaccttt aatttgctat ggatggccgg
     1981 ccttaaactc ctgatcgtcc tatttgcttc ctgagtgttg ggatcacaaa actgaaacaa
     2041 cacctccctc ctttggtctt gtgtggtact ggggatggaa ctgggtaaat gtgcatgcta
     2101 cacaagtact taaccacttg atccacatcc ctagtattat atgtaaggtg actcagagct
     2161 gagagatgga gaaaaaaaaa acggctatcc tgatgtgcag gggacactgg catttatcct
     2221 aaggtagggt gggggtgggg ggaaggttgc actcaaaaat agcttgtacc atacacacag
     2281 atagcagtta tacaattgtt ttgcatgtat gtgcacacat atgtgtgtat atgtgtgtgt
     2341 gtatttgttg ctctttggtt gatttgttta tttctctgaa tgtcctggaa cttgatctat
     2401 atagcctctg acaccagagt gctggaatta aacgtatgca ccatttccat ctggcttaaa
     2461 atggaacata tttagttatg tttacttaat gctgatgtct aagatgaaag aaagcattac
     2521 tatgatatta gctaattaaa atgtcctttg tattttattt agcttgtacc tgtgttcctg
     2581 agtgtgtctg agtctgtgca cctgagcatg tataggtcag aggtcacagg acacttgtag
     2641 gcagtctgtt cccggcttcc ttccattctg ggtggaaccg ggagatctaa tcaggtcacc
     2701 aggcttggct gcaggcccct tactcactga gccatctcac tgactccatg ttttattgta
     2761 ctaaatgtga tattcataca ttcatataac gtgtttatat taaattcact tcctccaatt
     2821 tgtccttcct aatttctcta ttttccctaa caacacaccc acgatttttc cttcctaact
     2881 tcctctttct ttatgttttg gattttattg ttgttactgt tcagttttag tttgacacag
     2941 gctctcactg tggctagtct cttgcaagtc tgttgctgag gtgggcctca aacttgggat
     3001 ccttctgccc tgcctcagtt tcctgagtac tggatggata tgcatcactg gctttggttt
     3061 cttcttctaa ctcttttcct tctctcctta gcatggaatt tgaagagctt catctgcttc
     3121 ttcctttagt cacagggaaa aagtggacta cacccagcct aggtgaagct ttaccggaga
     3181 cgcctcttgt tgaggtggcc agttgataaa gatgtaaatg ggattgggaa acaagaaacc
     3241 ctgtgtgtgc aggtgcagac cagtcattcc ttgttttgga gttggataca ggaagtacag
     3301 gaggtcaaaa tcgttttggg ctacttagtg agttcaagga ctttttgggt tacctaagac
     3361 cttgttcaaa ctaaacaagc ataatgctct ggagaagtgg cttggctcgt ggttccgtgg
     3421 tagaagcact ggtcagtttt gtagaggatc tggttttaga ttccagcacc cacgtggtgg
     3481 ctcaaaaccc atacatagct atagttccac aggatatgat gcccatttct gacctcgtgg
     3541 gcaccaggca cacacatggt acacatacat atatgcatgc ataactctct ctccccctct
     3601 ttgtatgtgt gtgtttatgt atatatatac acacacacat atacacacac agattaatct
     3661 tttttatata atgtattttt aattttcttc attagtgttg cctgcatgtt tgtctgtgtg
     3721 agggtgtcag atcctctgga actggagtta cagacagttg caagctgcca catggatgct
     3781 gggaattgaa ccctaggtcc tctggaagag caaccagtgc tcttaactgc tgagctgtct
     3841 ctccaaagtt aaagcaatgg gctggagata cagtgttggt tcacaagcat ctggaactcc
     3901 agatacaggc agctgggctg aagactctcc tttggcctct gtgggcaacg gacacatatc
     3961 cagtacatat tcatacgtgc aggcaacaca gccacacata taataagtac atagaaatta
     4021 aaaagaagtc aggcatggtg gtgcatgcct ttaatctcag cactctggag gcagaggcag
     4081 gcggatttct gagtttgagg ccagcctggt ctacacagtg agttccagga cagccagggc
     4141 tacacagaga aaccctgtct ggaaaaacca aaaaaaaaaa aaaaaaagat gagaagggga
     4201 aaaaacaagg aagaatccca ccccgcggtt tgattgcctt ttctggagtt aaggatgtta
     4261 gtgaatcacc atgtgaatgc tgggagcaac tcctgtgcca ggaatgctgc tctagccatt
     4321 gatccatctc tccagctcct tccctctgca gttcacactg aagtcctctt ctccttccta
     4381 ttcttgtgtg tgtccctatt cttcagtgaa tgtacccttg tgtgttattg atattgctgt
     4441 tattttgaaa cctctgtgca ggtctccctg gttagcctca aactccctct atagttaggg
     4501 atggtcttga actcctgctc cgtctgcttc catcttgtca aatactgcat gcactcctaa
     4561 gaccagcaaa ctgcagtaca gccaggacta catggtgaga ccctgtctca aaacaaaaca
     4621 aaacaaaaca aaacaaaaca aaacaaaaca aaacaaaaca aaactcatca cactgccgga
     4681 tgtgggggat caactggggc tcagagtaaa agtaggttct ttccatccac catgggtggg
     4741 ttctgggaat cgaactcggg tcatcaggct taatcacaat caatttgacc catggaatca
     4801 tcttactaac taacccttat tgaattttga gacaagggct tgctttccac acaggactga
     4861 gttcacattt accgcagagc tctgctagcc tctaaccact gagattccag gtgtgaccca
     4921 ctggatcttg ctgccttatt aacttttctt cctctctctg tctgtctgtc tctctctttc
     4981 tctcaggttt ttgttttttg tttttcctga gacagggttt ctctgtgtag ccctggctgt
     5041 cctggaacac actctgtaga ccaggctggc ctcaaactca gaaatccgtc tgcctctgcc
     5101 tcccaagtgc tgggattaaa ggcgtgcgcc accaccaccc agctcgggaa cagactgcct
     5161 ggtttctttt taacttttca tgtgtggcga gctgagtcaa aaatgtggtt ttctggatgc
     5221 caggcacact ctgagcgaca tgtttatact tgttctagtc ttctgttcct gtttcccccc
     5281 ttctcttact ttgtagcctt ggctggcttg caattttctt ggccaaccaa gatgctcctt
     5341 cttctgcctc ccaagtgcct ggaatatcca gcactatatc cagcatattc atatgtatat
     5401 tgaagactag gtggcctgtt atctttggaa caatacagag aaaattagca tgggccctat
     5461 gcaaggatga ccccaaagtt aatcaagtgt tccacatttt gccctttttc actctcatag
     5521 tcacctgcag ggaaactgag gtacaatgct atgaatctgc tgagccttat cactatcctt
     5581 ctgtcagctc cccaaatccc acccaccaag gtttttttaa aaaaacaaaa aacaaacaga
     5641 taacccaggc taggttatgt ttggctacaa acttgctatg tatgcaggaa tagaactcag
     5701 ggtttaaggc atgccacaca aacacactag gaatggagta gcagacccag tcacattctc
     5761 ttggaatcag tttagaacct gaataagaaa atgtctttgg gatcctgtag cggcaggagt
     5821 ttcctgagta tctttagtta ttctgaacaa tcctgttaat cgctggggag tttatgttaa
     5881 agagcttcag ggtggaacat ctgtagcctc tggggaaggg tagtctccaa aaggacagac
     5941 agatttactg gatttcaacc ttcacacctt tctaccaact tcctggaaag agggtggggg
     6001 gttcattggg atccctcaaa aaaaaaaaaa aaaaaaaaac cggcaggcag atcttttctg
     6061 agtcgctgag gctgagggaa ggatgtaggt ctggagagat cctggaagcc tcttgctgag
     6121 gctgagggaa ggatgtaggt ctggagagat cctggaagcc tcttgcatca tcatgctctg
     6181 catctgtcct ggctgagtgt cttcattctg ccaactctcg agatctgcga tctaagtaag
     6241 cttggcattc cggtactgtt ggtaaagcca ccatggaaga cgccaaaaac ataaagaaag
     6301 gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta
     6361 tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg
     6421 acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat
     6481 atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta
     6541 tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata
     6601 atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca
     6661 aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta
     6721 ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat
     6781 ctcatctacc tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca
     6841 agacaattgc actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc
     6901 tgcctcatag aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc
     6961 aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt
     7021 ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag
     7081 aagagctgtt tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa
     7141 ccctattctc cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac
     7201 acgaaattgc ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga
     7261 ggttccatct gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc
     7321 tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg
     7381 aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac
     7441 tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg
     7501 ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg
     7561 aacacttctt catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg
     7621 ctcccgctga attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg
     7681 caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg
     7741 gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga
     7801 aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac
     7861 tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg
     7921 tgtaattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg
     7981 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     8041 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     8101 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     8161 acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt gagagccttc
     8221 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     8281 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct
     8341 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8401 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8461 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8521 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8581 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8641 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8701 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8761 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8821 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8881 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8941 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9001 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9061 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9121 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9181 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9241 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9301 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9361 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9421 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9481 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9541 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9601 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9661 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9721 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9781 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9841 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9901 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9961 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10021 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10081 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10141 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10201 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc
    10261 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    10321 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    10381 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    10441 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
    10501 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    10561 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    10621 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    10681 gaattttaac aaaatattaa cgtttacaat ttcccattcg ccattcaggc tgcgcaactg
    10741 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagcccaagc taccatgata
    10801 agtaagtaat attaaggtac gggaggtact tggagcggcc gcaataaaat atctttattt
    10861 tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat
    10921 caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt
    10981 gccagaacat ttctctatcg ata
//
