LOCUS       sequence-106631-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse 
            beta-Catenin. Expresses Cas9 from CBh promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Xue W, Chen S, Yin H, Tammela T, Papagiannakopoulos T, Joshi NS, Cai
            W, Yang G, Bronson R, Crowley DG, Zhang F, Anderson DG, Sharp PA, 
            Jacks T
  TITLE     CRISPR-mediated direct mutation of cancer genes in the mouse liver.
  JOURNAL   Nature. 2014 Aug 6. doi: 10.1038/nature13589.
  PUBMED    25119044
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Aug 6. doi: 10.1038/nature13589."
COMMENT     Sequence Label: pX330 Ctnnb1.1
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             62..127
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             134..154
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggaccg gtgccaccat
       61 ggactataag gaccacgacg gagactacaa ggatcatgat attgattaca aagacgatga
      121 cgataagatg gccccaaaga agaagcggaa ggtcggtatc cacggagtcc cagcagccga
      181 caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg ccgtgatcac
      241 cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag
      301 catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac
      361 ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct
      421 gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga
      481 agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat
      541 cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact
      601 ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat
      661 caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga
      721 caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa
      781 cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct
      841 ggaa
//
