LOCUS       sequence-106635-        6147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter plasmid used to study transcriptional control of
            murine Tet2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Sohni A, Bartoccetti M, Khoueiry R, Spans L, Vande Velde J, De 
            Troyer L, Pulakanti K, Claessens F, Rao S, Koh KP
  TITLE     Dynamic switching of active promoter and enhancer domains regulates 
            Tet1 and Tet2 expression during cell state transitions between 
            pluripotency and differentiation.
  JOURNAL   Mol Cell Biol. 2015 Mar 15;35(6):1026-42. doi: 10.1128/MCB.01172-14.
            Epub 2015 Jan 12.
  PUBMED    25582196
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01172-14"; journalName: "Mol Cell Biol"; date: 
            "2015-03-15- 15"; volume: "35"; issue: "6"; pages: "1026-42"
COMMENT     Sequence Label: pGL3-SV40P-Tet2 enhancer H1
FEATURES             Location/Qualifiers
     source          1..6147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..233
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      84..219
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      263..272
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             269..1921
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(335..353)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    1962..2083
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1973..1992)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2027..2046
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3479..3496)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3650..4238)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3730..3749)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4409..5200,5201..5269))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5032..5051
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5270..5374)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5401..5856
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5488..5507)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5698..5719
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5987..6035
                     /note="synthetic polyadenylation signal"
     misc_feature    6049..6140
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6089..6108
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctaggatct gcgatctgca tctcaattag tcagcaacca
       61 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
      121 cgccccatcg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
      181 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
      241 gcattccggt actgttggta aagccaccat ggaagacgcc aaaaacataa agaaaggccc
      301 ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa
      361 gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat
      421 cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg
      481 gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc
      541 ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga
      601 acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa
      661 ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat
      721 catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca
      781 tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac
      841 aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc
      901 tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat
      961 cattccggat actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac
     1021 tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga
     1081 gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct
     1141 attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga
     1201 aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt
     1261 ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat
     1321 tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc
     1381 gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg
     1441 tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt
     1501 gattgacaag gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca
     1561 cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc
     1621 cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg
     1681 tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa
     1741 gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa
     1801 gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga
     1861 cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta
     1921 attctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca ttgatgagtt
     1981 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     2041 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     2101 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     2161 ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccaga gcgagcagag gtcctgaatt
     2221 caattcccag caaccacatg atggctcaac catcagtaca gctactgtat actcatatac
     2281 ataaaataaa ataaataaat ctttttcaaa aagggttttg cctgcatgta tgtatgtgca
     2341 ccatgtgcat gcccggtgct tgaagcaacc aaaagaggat gtcatatctc tcagaatgta
     2401 agttatggat tattgtgata ttacatgtgg tagattgaac cccaaacctc tggtaagacc
     2461 tgccagtgct tttaagcact gagcccttca gcaccagtat atttaatttt aaagtgattt
     2521 aaataaacaa gaaagaataa attaaatcct tcttagagca attttgtcat gcacatgtac
     2581 atttttatgg ccaaggaaat aggtttctgt gtgcaaaaga agcagaggag actattacac
     2641 agttagacac gtggacctgg gctctcagca gagctattta cactttgcca acctccaaga
     2701 gagatggctt ttgtgtctgg ggccagatct ccaccccctt aattaatagt caaaaatctc
     2761 ctgtcctggc tctgaccggg tcatgacccc aagatcaagg tgtttaggtg agacctgccc
     2821 aattcaaagt ttatttgcgg aagcctggcc tgctgagcct gccttggctt aggttaatct
     2881 tttgtgtctc ccgaggcttg cggagccggt cttgctggcc tttttgtttg tttttgtagg
     2941 tgatgtcttg tttctcttaa ttacagtgtc cagtgcatta agtatgatcc cttctcacaa
     3001 atgcttttca aataacaatc agcagtgaca aagggggatc ttggaggagc tgagggaggg
     3061 ggaatcataa tcagaacata gtgtatgaaa aaaatccatt tcaacttaaa agaagaagga
     3121 gaaggaaatc ctttttcatt ttggggtctc ggtgatccta cttcccaaag caggaactat
     3181 ggccacagga aagttgttgc ttaccaagcc gctctcccca cctccacact ggcatctgaa
     3241 atcctcctgt tggatgtctg gcccgcccta cccgggaccc tcctcaccct tgggggttaa
     3301 cacaatagaa agctttcact tgggacttcc tgctttccgt cgaccgatgc ccttgagagc
     3361 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     3421 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct
     3481 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3541 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3601 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3661 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3721 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3781 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3841 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3901 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3961 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4021 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4081 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4141 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4201 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4261 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4321 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4381 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4441 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4501 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4561 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4621 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4681 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     4741 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4801 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4861 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     4921 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     4981 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5041 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5101 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5161 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5221 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     5281 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5341 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     5401 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5461 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5521 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5581 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5641 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5701 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5761 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5821 acgcgaattt taacaaaata ttaacgttta caatttccca ttcgccattc aggctgcgca
     5881 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat
     5941 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt
     6001 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct
     6061 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc
     6121 aggtgccaga acatttctct atcgata
//
