LOCUS       sequence-106637-         542 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. 
            Expresses Cas9 from CBh promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Xue W, Chen S, Yin H, Tammela T, Papagiannakopoulos T, Joshi NS, Cai
            W, Yang G, Bronson R, Crowley DG, Zhang F, Anderson DG, Sharp PA, 
            Jacks T
  TITLE     CRISPR-mediated direct mutation of cancer genes in the mouse liver.
  JOURNAL   Nature. 2014 Aug 6. doi: 10.1038/nature13589.
  PUBMED    25119044
REFERENCE   2  (bases 1 to 542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Aug 6. doi: 10.1038/nature13589."
COMMENT     Sequence Label: pX330 Pten
FEATURES             Location/Qualifiers
     source          1..542
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        123..371
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     123..143
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     294..313
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        393..468
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
       61 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      121 gtgagggcct atttcccatg attccttcat atttgcatat acgatacaag gctgttagag
      181 agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta
      241 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
      301 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
      361 gacgaaacac cgagatcgtt agcagaaaca aagttttaga gctagaaata gcaagttaaa
      421 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgtttta
      481 gagctagaaa tagcaagtta aaataaggct agtccgtttt tagcgcgtgc gccaattctg
      541 ca
//
