LOCUS       sequence-106652-         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. 
            Expresses Cas9 from CBh promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Xue W, Chen S, Yin H, Tammela T, Papagiannakopoulos T, Joshi NS, Cai
            W, Yang G, Bronson R, Crowley DG, Zhang F, Anderson DG, Sharp PA, 
            Jacks T
  TITLE     CRISPR-mediated direct mutation of cancer genes in the mouse liver.
  JOURNAL   Nature. 2014 Aug 6. doi: 10.1038/nature13589.
  PUBMED    25119044
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Aug 6. doi: 10.1038/nature13589."
COMMENT     Sequence Label: pX330 p53
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..57)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gaattcttac tttttctttt ttgcctggcc ggcctttttc gtggccgccg gccttttgtc
       61 gcctcccagc tgagacaggt cgatccgtgt ctcgtacagg ccggtgatgc tctggtggat
      121 cagggtggcg tccagcacct ctttggtgct ggtgtacctc ttccggtcga tggtggtgtc
      181 aaagtacttg aaggcggcag gggctcccag attggtcagg gtaaacaggt ggatgatatt
      241 ctcggcctgc tctctgatgg gcttatcccg gtgcttgttg taggcggaca gcactttgtc
      301 cagattagcg tcggccagga tcactctctt ggagaactcg ctgatctgct cgatgatctc
      361 gtccaggtag tgcttgtgct gttccacaaa cagctgtttc tgctcattat cctcggggga
      421 gcccttcagc ttctcatagt ggctggccag gtacaggaag ttcacatatt tggagggcag
      481 ggccagttcg tttcccttct gcagttcgcc ggcagaggcc agcattctct tccggccgtt
      541 ttccagctcg aacagggagt acttaggcag cttgatgatc aggtcctttt tcacttcttt
      601 gtagcccttg gcttccagaa agtcgatggg attcttctcg aagctgcttc tttccatgat
      661 ggtgatcccc agcagctctt tcacactct
//
