LOCUS       sequence-106687-        5085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recipient replicative vector to construct MYC tagged fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, 
            Patron-Soberano A, De Las Penas A, Castano I
  TITLE     Expression vectors for C-terminal fusions with fluorescent proteins 
            and epitope tags in Candida glabrata.
  JOURNAL   Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020.
  PUBMED    25986172
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020."
COMMENT     Sequence Label: pYC66
FEATURES             Location/Qualifiers
     source          1..5085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        341..747
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             761..1333
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      1340..1587
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(1356..1374)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     1608..1624
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1609..1625
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1681..2178
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     primer_bind     complement(2288..2308)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2288..2306)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2327..2343)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2327..2343)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2340..2362)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2351..2367
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2375..2381,2382..2399,2400..2405))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2420..2441
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2558..2575)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2729..3317)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2809..2828)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3488..4279,4280..4348))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4111..4130
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4349..4420)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5058..5076
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcacctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca taccataaag cacagctcca tagcttcaaa
      361 atgtttctac tcctttttta ctcttccaga ttttctcgga ctccgcgcat cgccgtacca
      421 cttcaaaaca cccaagcaca gcatactaaa tttcccctct ttcttcctct agggtgtcgt
      481 taattacccg tactaaaggt ttggaaaaga aaaaagagac cgcctcgttt ctttttcttc
      541 gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt cttgaaaatt ttttttttga
      601 tttttttctc tttcgatgac ctcccattga tatttaagtt aataaacggt cttcaatttc
      661 tcaagtttca gtttcatttt tcttgttcta ttacaacttt ttttacttct tgctcattag
      721 aaagaaagca tagcaatcta atctaagttt tggatctaaa atgggcacca ctcttgacga
      781 cacggcttac cgataccgca ccagtgtccc gggggacgcc gaggccatcg aggcactgga
      841 tgggtccttc accaccgaca ccgtcttccg cgtcaccgcc accggggacg gcttcaccct
      901 gcgggaggtg ccggtggacc cgcccctgac caaggtgttc cccgacgacg aatcggacga
      961 cgaatcggac gacggggagg acggcgaccc tgactcccgg acgttcgtcg cgtacgggga
     1021 cgacggcgac ctggcgggct tcgtggtcgt ctcgtactcc ggctggaacc gccggctgac
     1081 cgtcgaggac atcgaggtcg ccccggagca ccgggggcac ggggtcgggc gcgcgttgat
     1141 ggggctcgcg acggagttcg cccgcgagct gggcgccggg cacctctggc tggaggtcac
     1201 caacgtcaac gcaccggcga tccacgcgta ccggcggatg gggttcaccc tctgcggcct
     1261 ggacaccgcc ctgtacgacg gcaccgcctc ggacggcgag caggcgctct acatgagcat
     1321 gccctgcccc taagtcgagt catgtaatta gttatgtcac gcttacattc acgccctccc
     1381 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt
     1441 tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
     1501 tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt
     1561 ttgggacgct cgaaggcttt aatttgcggc cggtaccggg ccccccctcg aggtcgacgg
     1621 tatcgataag cttgatatcg aattcctgca gcccggggga tcctagggtt aattaacggt
     1681 gaacaaaagc taatctccga ggaagacttg aacggtgaac aaaaattaat ctcagaagaa
     1741 gacttgaacg gactcgacgg tgaacaaaag ttgatttctg aagaagattt gaacggtgaa
     1801 caaaagctaa tctccgagga agacttgaac ggtgaacaaa aattaatctc agaagaagac
     1861 ttgaacggac tcgacggtga acaaaagttg atttctgaag aagatttgaa cggtgaacaa
     1921 aagctaatct ccgaggaaga cttgaacggt gaacaaaaat taatctcaga agaagacttg
     1981 aacggactcg acggtgaaca aaagttgatt tctgaagaag atttgaacgg tgaacaaaag
     2041 ctaatctccg aggaagactt gaacggtgaa caaaaattaa tctcagaaga agacttgaac
     2101 ggactcgacg gtgaacaaaa gttgatttct gaagaagatt tgaacggtga acaaaagcta
     2161 atctccgagg aagacttgaa cggtgaacaa aaattaatca atcactagct agtgaattcg
     2221 cgccagatcc gaagttccta tactttctag agcggccgcc accgcggtgg agctccagct
     2281 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
     2341 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     2401 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     2461 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     2521 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2581 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2641 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2701 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2761 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2821 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2881 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2941 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3001 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3061 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3121 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     3181 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3241 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3301 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3361 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3421 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3481 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3541 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3601 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3661 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3721 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3781 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3841 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3901 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3961 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4021 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4081 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4141 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4201 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4261 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4321 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4381 atcagggtta ttgtctcatg agcggataca tatttgaatg tggggatctc taatgatgca
     4441 atttttgttt tgtacactaa gattctattt cggaactctt acccatgtca ttatttactt
     4501 tattttcatt attgtaattt tttaagcttt tattatgaat tatttaaata caattctttt
     4561 tgatcatgtg acagacatca ctgaaagatg tgctctgcgt tattagtcag attgaaatat
     4621 acattttcat gtagatatga tgttcttact ttaaatgttg ttccatttgc tagcttaaat
     4681 agacgatatt ataaatttaa gtattcacta aaaatctgac ccattaatgt tagaattagt
     4741 attatttttt gtgatattac atttttatat tttggatttc aaccaatgct ctgcgatatg
     4801 attcaacaga ataatatgta caagctactt taattcatat atactaatag attcacagat
     4861 ccagtacgac tgcaagaatt tacgttttgt ttgaaatggt agtagctaca ctaaattaac
     4921 aatgatcgat taatccacat ccacagtgtg taatcatcct aatgaaaaca agaataacta
     4981 caaaaatgaa tataaacttt ttgcatgtta gtctaacctg ctaagaaacc attattatca
     5041 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
