LOCUS       sequence-106689-         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV 
            promoter and mCherry module. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV mCherry L1-R5
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(177..196)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(178..196)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(241..444)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(270..290)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac
       61 tccttgatga tggccatgtt atcctcctcg cccttgctca ccatggtaag ctttttgcaa
      121 aagcctaggc ctccaaaaaa gcctccggat ccgagctcgg taccaagctt gggtctccct
      181 atagtgagtc gtattaattt cgataagcca gtaagcagtg ggttctctag ttagccagag
      241 agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc
      301 ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt
      361 gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt
      421 gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg
      481 ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc
      541 gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg
      601 ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac
      661 tatgggaaca tacgtcatta ttgacgtc
//
