LOCUS       sequence-106700-         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  const: miRFKBP5_GFP_IRES_Puro; ind: mC-FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB122
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    111..129
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    146..164
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    182..200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    218..236
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    253..271
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    289..307
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        319..357
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     354..378
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     ncRNA           535..561
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           587..628
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 acgctcgctg cgcccttcgt ctgacgtggc agcgctcgcc gtgaggaggg gggcgcccgc
       61 gggaggcgcc aaaacccggc gcggaggcca cgcgtactag acgagtttac tccctatcag
      121 tgatagagaa cgatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
      181 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
      241 atgtcgagtt tatccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
      301 atagagaacg tatgtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt
      361 ttagtgaacc gtcagatcgc accgggtgac tctcttaagg tagccttgca gaagttggtc
      421 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      481 actgggcttg tcgagacaga gaagactctt gcgtttcggt cgacttctag agctctggag
      541 gcttgctgaa ggctgtatgc tagagacgta cagatgcgtc tcacaggaca caaggcctgt
      601 tactagcact cacatggaac aaatggccga attcggcgct agctgctgat atcgcatacg
      661 cgtggaccag ataggcacct attggtctta ctgacat
//
