LOCUS       sequence-106701-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  const: miRFKBP5_GFP_IRES_Puro; ind: mC-FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB122
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(139..158)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     ncRNA           complement(233..274)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(300..326)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(483..507)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(504..542)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    554..572
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    590..608
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    625..643
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    661..679
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac
       61 tccttgatga tggccatgtt atcctcctcg cccttgctca ccatggtgac cggtggatcc
      121 cgggcccgcg gtaccgacac ctgtggagag aaaggcaaag tggatgtcag taagaccaat
      181 aggtgcctat ctggtccacg cgtatgcgat atcagcagct agcgccgaat tcggccattt
      241 gttccatgtg agtgctagta acaggccttg tgtcctgtga gacgcatctg tacgtctcta
      301 gcatacagcc ttcagcaagc ctccagagct ctagaagtcg accgaaacgc aagagtcttc
      361 tctgtctcga caagcccagt ttctattggt ctccttaaac ctgtcttgta accttgatac
      421 ttacctgccc agtgcctcac gaccaacttc tgcaaggcta ccttaagaga gtcacccggt
      481 gcgatctgac ggttcactaa acgagctctg cttatatagg cctcccaccg tacacgccta
      541 cctcgacata cgttctctat cactgatagg gagtaaactc gacatacgtt ctctatcact
      601 gatagggata aactcgacat acgttctcta tcactgatag ggagtaaact cgacatacgt
      661 tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat a
//
