LOCUS       sequence-106705-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutive miRFKBP5_Mis12-GFP-FKBP3; inducible mCh-FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB109
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    188..206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    224..242
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    259..277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    295..313
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        325..363
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     360..384
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     ncRNA           541..567
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           593..634
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     709..728
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 atcaggacgc tcgctgcgcc cttcgtctga cgtggcagcg ctcgccgtga ggaggggggc
       61 gcccgcggga ggcgccaaaa cccggcgcgg aggccacgcg tactagacga gtttactccc
      121 tatcagtgat agagaacgat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
      241 gaacgtatgt cgagtttatc cctatcagtg atagagaacg tatgtcgagt ttactcccta
      301 tcagtgatag agaacgtatg tcgaggtagg cgtgtacggt gggaggccta tataagcaga
      361 gctcgtttag tgaaccgtca gatcgcaccg ggtgactctc ttaaggtagc cttgcagaag
      421 ttggtcgtga ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca
      481 atagaaactg ggcttgtcga gacagagaag actcttgcgt ttcggtcgac ttctagagct
      541 ctggaggctt gctgaaggct gtatgctaga gacgtacaga tgcgtctcac aggacacaag
      601 gcctgttact agcactcaca tggaacaaat ggccgaattc ggcgctagct gctgatatcg
      661 catacgcgtg gaccagatag gcacctattg gtcttactga catccacttt gcctttctct
      721 ccacaggtgt cggtaccgc
//
