LOCUS       sequence-106715-        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of a germ-line targeted, codon optimized Cas9 for 
            genome-editing in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Moreno-Mateos MA, Vejnar CE, Beaudoin JD, Fernandez JP, Mis EK, 
            Khokha MK, Giraldez AJ
  TITLE     CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 
            targeting in vivo.
  JOURNAL   Nat Methods. 2015 Aug 31. doi: 10.1038/nmeth.3543.
  PUBMED    26322839
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Aug 31. doi: 10.1038/nmeth.3543."
COMMENT     Sequence Label: pCS2-nCas9n-nanos 3'UTR
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(684..704)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     RBS             799..807
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gtctggatct acgtaatacg actcactata gttctagatg tttatatttt cctcacattt
       61 ttcactccat cacaagtcaa aaatcacaca ctgacataaa atgatgaaag tagtacatga
      121 attttagcaa gcgacatgaa aatatggcga ttatgttttt caagctgaat ttaaatgcaa
      181 tggagaaaac tttttttaaa ggttacttat acaaaacaag ccgttttcaa taataaaatc
      241 actaaaatct aaatattaac attgaatttt ttgtttaaat acaacaccat aaaatctgac
      301 atttaaaagg aggactaaac acacattgta tttaccaaat aagcatgaaa gggtttgttt
      361 ttgagtgcgg ttgcgcctgt agcgagcctg ctggcttgtg tacgagtttg ttaccaagtg
      421 aaaacacaac accagtgcac acaaaataaa acacagcaaa cacacacata tacacacaca
      481 caaaaaaagt gcaaaatcaa acagtgaacg cacacatgca tgcaaactcg caatccgccg
      541 cgtatcaacc aaacaaacaa ttaaagagtt aaaacttaaa cattaaaacc tgcggtaaaa
      601 agtgtttctt caaatctccc ggagcatcaa tgtccgctac cggtgagtaa tacgactcac
      661 tatagttcta gattccgcgg tcacaccttt ctcttcttct taggagatct caccggtgag
      721 tcgccaccca gctgagacag gtcaatcctt gtttcgtaca ggccggtaat actctgatgg
      781 atcagtgtag cgtccagcac ctccttagtg gaggtgtacc tctttctatc gattgtagtg
      841 tcgaagtact tgaaagctgc aggtgcgccc aggtttgtca gagtgaacag atgaatgata
      901 ttttcggcct gctccctgat gggcttatct ctgtgtttgt tgtaagcaga caggacctta
      961 tccagatttg cgtcggccag gatcactctt ttagagaact cgctaatctg ttcaatgatc
     1021 tcgtccagat aatgcttgtg ctgctccaca aacagctgtt tctgttcgtt atcctctgga
     1081 gatcctttca gcttctcgt
//
