LOCUS       sequence-106735-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human Alpha7 (CHRNA7) with YFP fused into 
            M3-M4 loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Wang Y, Xiao C, Indersmitten T, Freedman R, Leonard S, Lester HA
  TITLE     The duplicated alpha7 subunits assemble and form functional 
            nicotinic receptors with the full-length alpha7.
  JOURNAL   J Biol Chem. 2014 Sep 19;289(38):26451-63. doi: 
            10.1074/jbc.M114.582858. Epub 2014 Jul 23.
  PUBMED    25056953
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.582858"; journalName: "J Biol Chem"; date: 
            "2014-09-19- 19"; volume: "289"; issue: "38"; pages: "26451-63"
COMMENT     Sequence Label: pcDNA3.1-CHRNA7-YFP
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             123..374
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     311..332
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
       61 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      121 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      181 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      241 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
      301 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      361 acgagctgta caaggcaggc gcctccccca cgcacgatga gcacctcctg cacggtgggc
      421 aaccccccga gggggacccg gacttggcca agatcctgga ggaggtccgc tacattgcca
      481 accgcttccg ctgccaggac gaaagcgagg cggtctgcag cgagtggaag ttcgccgcct
      541 gtgtggtgga ccgcctgtgc ctcatggcct tctcggtctt caccatcatc tgcaccatcg
      601 gcatcctgat gtcggctccc aacttcgtgg aggccgtgtc caaagacttt gcgtaaccac
      661 gcctggttct gtacatgtgg aaaactcaca gatgggcaag gcctttggct tggcgagatt
      721 tgggggtgct aatccaggac agcattacac gccacaactc cagtgttccc ttctggctgt
      781 cagtcgtgtt gcttacggtt tctttgttac tttaggtagt agaatctcag cactttgttt
      841 catattctca gatgggctga tagatatcct tggcacatcc gtaccatcgg tcagcagggc
      901 cactgagtag tcattttgcc cattagccca ctgcctggaa agcccttcgg agagctcccc
      961 atggctcctc accaccgaga cagttggttt tgcatgtctg catgaaggtc tacctgaaa
//
