LOCUS       sequence-106736-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (OsUBI promoter) for direct cloning of 
            artificial microRNAs into rice MIR390 precursor. To use for 
            expressing amiRNAs in monocots..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Carbonell A, Fahlgren N, Mitchell S, Cox KL Jr, Mockler TC, 
            Carrington JC
  TITLE     Highly Specific Gene Silencing in a Monocot Species by Artificial 
            MicroRNAs Derived From Chimeric MIRNA Precursors.
  JOURNAL   Plant J. 2015 Mar 24. doi: 10.1111/tpj.12835.
  PUBMED    25809382
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2015 Mar 24. doi: 10.1111/tpj.12835."
COMMENT     Sequence Label: pH7WG2B-OsMIR390-B/c
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             373..678
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     557..576
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta aacgcgtgga gccggcttac taaaagccag
       61 ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta
      121 tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac
      181 agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca
      241 caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg
      301 aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca
      361 ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg
      421 tttgtggatg tacagagtga tattattgac acgcccggcc gacggatggt gatccccctg
      481 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc
      541 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ttccgttatc
      601 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
      661 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc acctcgacgg
      721 tctcacat
//
