LOCUS       sequence-106737-         639 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (OsUBI promoter) for direct cloning of 
            artificial microRNAs into rice MIR390 precursor. To use for 
            expressing amiRNAs in monocots..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Carbonell A, Fahlgren N, Mitchell S, Cox KL Jr, Mockler TC, 
            Carrington JC
  TITLE     Highly Specific Gene Silencing in a Monocot Species by Artificial 
            MicroRNAs Derived From Chimeric MIRNA Precursors.
  JOURNAL   Plant J. 2015 Mar 24. doi: 10.1111/tpj.12835.
  PUBMED    25809382
REFERENCE   2  (bases 1 to 639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2015 Mar 24. doi: 10.1111/tpj.12835."
COMMENT     Sequence Label: pH7WG2B-OsMIR390-B/c
FEATURES             Location/Qualifiers
     source          1..639
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(85..91,92..109,110..115))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(200..224)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
       61 acggctccta actcaaaatc cacacattat acgagccgga agcataaagt gtaaagcctg
      121 gggtgcctaa tggtctctca aggattgttc catacccttc ctcaaaacat ctcgagctcg
      181 gtgaaggggg cggccgcgga gcctgctttt ttgtacaaac ttgtgatatc actagcaagc
      241 ttgggcccga tctgaatctg caagaaataa tcaccaaaca gatagggtaa gaactagcac
      301 aagattaagg acacaacaac ggatcaaaca gcaaacatat tccaacagaa catacatgta
      361 ttcaaaaaga tagtaacagg cacatcacac atgtattcag ataggtagta acagcacacg
      421 tatcctgaac ttcagaaata agtagaatga actagtggca ataatcttca cacagatagt
      481 aaaaagaaat taccacaata gattatatag atgaactaca gattgtaaca tgatattact
      541 acagattgta acatgatatt ctaatatagt tcttagcata aattactggc agatagtaac
      601 atgagatcat tatgacagat atagcgacaa gacttccta
//
