LOCUS       sequence-106752-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human duplicated Alpha7-2 bp deletion 
            (CHRFAM7A-∆2bp) with mEGFP fused into M3-M4 loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Wang Y, Xiao C, Indersmitten T, Freedman R, Leonard S, Lester HA
  TITLE     The duplicated alpha7 subunits assemble and form functional 
            nicotinic receptors with the full-length alpha7.
  JOURNAL   J Biol Chem. 2014 Sep 19;289(38):26451-63. doi: 
            10.1074/jbc.M114.582858. Epub 2014 Jul 23.
  PUBMED    25056953
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.582858"; journalName: "J Biol Chem"; date: 
            "2014-09-19- 19"; volume: "289"; issue: "38"; pages: "26451-63"
COMMENT     Sequence Label: pcDNA3.1-CHRFAM7ADelta2bp-mGFP
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             123..374
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     311..332
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(777..794)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    783..1007
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
       61 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      121 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      181 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      241 tgctgcccga caaccactac ctgagcaccc agtccaagct tagcaaagac cccaacgaga
      301 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      361 acgagctgta caaggcaggc gcctccccca cgcacgatga gcacctcctg cacggcgggc
      421 aaccccccga gggggacccg gacttggcca agatcctgga ggaggtccgc tacattgcca
      481 accgcttccg ctgccaggac gaaagcgagg cggtctgcag cgagtggaag ttcgccgcct
      541 gtgtggtgga ccgcctgtgc ctcatggcct tctcggtctt caccatcatc tgcaccatcg
      601 gcatcctgat gtcggctccc aacttcgtgg aggccgtgtc caaagacttt gcgtaaccac
      661 gcctggttct gtacatgtgg aaaactcaca gatgggcaag gaagggcaat tctgcagata
      721 tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc
      781 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
      841 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
      901 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
      961 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     1021 aagaaccagc tggg
//
