LOCUS       sequence-106754-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RPL16a HA-TEV-AVI pop-in/pop-out plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Jan CH, Williams CC, Weissman JS
  TITLE     Principles of ER cotranslational translocation revealed by 
            proximity-specific ribosome profiling.
  JOURNAL   Science. 2014 Nov 7;346(6210):1257521. doi: 10.1126/science.1257521.
            Epub 2014 Nov 6.
  PUBMED    25378630
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2014-11-7"; volume: "346(6210):1257521. doi"; pages: " 
            10.1126/science.1257521. Epub 2014 Nov 6"
COMMENT     Sequence Label: pJW1505
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     47..63
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     48..64
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             547..573
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             583..603
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             607..651
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga ggtcgacggt
       61 atcgataagc ttgatatcga attcctgcag cccgggggat ccactagtga tttcttgaga
      121 aaggctaccg ctttcaataa gacccgtggt ccattccatt tcagagcccc atctagaatc
      181 ttctacaaag ctcttcgtgg tatggtttct cacaagactg ctcgtggtaa ggccgctttg
      241 gaacgtttaa aggtctttga aggtatccca cctccatacg ataagaagaa gagagttgtt
      301 gtcccacaag cattgagagt tttgagattg aagccaggta gaaagtacac caccttgggt
      361 aagttgtcta cttccgttgg ttggaaatac gaagatgttg ttgccaaatt ggaagcaaag
      421 agaaaggttt catccgctga atactatgcc aagaagagag ctttcaccaa gaaagttgcc
      481 tctgctaatg ctactgctgc tgaatctgat gttgctaaac aattagccgc tttgggttac
      541 ggttcttacc catacgacgt cccagactac gctggaggtt cagaaaattt gtattttcaa
      601 ggatcaggtt tgaatgatat ttttgaagct caaaaaattg aatggcatga gtaagaaatt
      661 aaaaatagtt tttctttaat actttacatt ttgtaatata atcacaaata ttttttagaa
      721 ataataaaaa ccaaagaaac cattcctccc cgttacagtt tagattacct cccctttttc
      781 tttcgtactt t
//
