LOCUS       sequence-106758-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS316-pTDH3-Su9-TagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Williams CC, Jan CH, Weissman JS
  TITLE     Targeting and plasticity of mitochondrial proteins revealed by 
            proximity-specific ribosome profiling.
  JOURNAL   Science. 2014 Nov 7;346(6210):748-51. doi: 10.1126/science.1257522.
  PUBMED    25378625
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-11-7- 7"; volume: "346"; issue: 
            "6210"; pages: "748-51"
COMMENT     Sequence Label: pJW1513
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    31..138
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     47..63
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     48..64
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(98..114)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(98..114)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     682..705
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga ggtcgacggt
       61 atcgataagc ttgatatcga attcctgcag cccgggggat ccactagttc tagagcggcc
      121 gccaccgcgg tggagctcag tttatcatta tcaatactcg ccatttcaaa gaatacgtaa
      181 ataattaata gtagtgattt tcctaacttt atttagtcaa aaaattagcc ttttaattct
      241 gctgtaaccc gtacatgccc aaaatagggg gcgggttaca cagaatatat aacatcgtag
      301 gtgtctgggt gaacagttta ttcctggcat ccactaaata taatggagcc cgctttttaa
      361 gctggcatcc agaaaaaaaa agaatcccag caccaaaata ttgttttctt caccaaccat
      421 cagttcatag gtccattctc ttagcgcaac tacagagaac aggggcacaa acaggcaaaa
      481 aacgggcaca acctcaatgg agtgatgcaa cctgcctgga gtaaatgatg acacaaggca
      541 attgacccac gcatgtatct atctcatttt cttacacctt ctattacctt ctgctctctc
      601 tgatttggaa aaagctgaaa aaaaaggttg aaaccagttc cctgaaatta ttcccctact
      661 tgactaataa gtatataaag acggtaggta ttgattgtaa ttctgtaaat ctatttctta
      721 aacttcttaa attctacttt tatagttagt cttttt
//
