LOCUS       sequence-106761-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human duplicated Alpha7-2 bp deletion 
            (CHRFAM7A-∆2bp) with mEGFP fused into M3-M4 loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Wang Y, Xiao C, Indersmitten T, Freedman R, Leonard S, Lester HA
  TITLE     The duplicated alpha7 subunits assemble and form functional 
            nicotinic receptors with the full-length alpha7.
  JOURNAL   J Biol Chem. 2014 Sep 19;289(38):26451-63. doi: 
            10.1074/jbc.M114.582858. Epub 2014 Jul 23.
  PUBMED    25056953
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.582858"; journalName: "J Biol Chem"; date: 
            "2014-09-19- 19"; volume: "289"; issue: "38"; pages: "26451-63"
COMMENT     Sequence Label: pcDNA3.1-CHRFAM7ADelta2bp-mGFP
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     225..246
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
       61 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
      121 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
      181 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
      241 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggcgccagc
      301 gcaggccatg cggccacaca ctaccccaga gtcgggggtc gggacacagt gcacgccgtc
      361 caggccgcgg aagccgatgt acagcaggtt cccgttgctg gcgggcggcg gcgccacggc
      421 gctcatctcc acactggcca ggctgcagcg ccgctgcttg tgctggcagg ccgggcgcac
      481 cttgtcctcc ccgggcctct tcattcgcag gaaccacgcg caccagttca gaaggatgac
      541 tctggtccac ttgggcatct tgcccccgtc ggggtcgtgg tggtggtact gcagcacgat
      601 caccgtcacc accaccgaga ggcccacgat gatcatggtg ctggcgaagt actgggctat
      661 caatggtacc gaatcggatg ttgcgggcat gatctcagcc acgagcagca tgaagacggt
      721 aagagagagt aagactgtta tccccaggga aatcttctcc ccggaatctg caggaagcag
      781 gaacaccagc agggcgaggg cggagatgag cacacagggg atcagcaggt tgaggccata
      841 gtagagtgtc ctgcggcgca tggtcactgt gaaggtgaca tcagggtagg gctctttgca
      901 gcactcatag aacctttcac tcctcttgcc ggggattccc actaggtccc attctccatt
      961 ggggatatag ccactgatat ctgcctcctg catctgcaga tccaaggacc agcctccgta
     1021 agaccaggac ccaaacttgt ttgcagtgct gcacatcaa
//
