LOCUS       sequence-106763-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human duplicated Alpha7-2 bp deletion 
            (CHRFAM7A-∆2bp) with YFP fused into M3-M4 loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Wang Y, Xiao C, Indersmitten T, Freedman R, Leonard S, Lester HA
  TITLE     The duplicated alpha7 subunits assemble and form functional 
            nicotinic receptors with the full-length alpha7.
  JOURNAL   J Biol Chem. 2014 Sep 19;289(38):26451-63. doi: 
            10.1074/jbc.M114.582858. Epub 2014 Jul 23.
  PUBMED    25056953
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.582858"; journalName: "J Biol Chem"; date: 
            "2014-09-19- 19"; volume: "289"; issue: "38"; pages: "26451-63"
COMMENT     Sequence Label: pcDNA3.1-CHRFAM7ADelta2bp-YFP
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     128..147
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     387..408
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
       61 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
      121 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
      181 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa
      241 gcactgcagg ccgtagccga aggtggtcac gagggtgggc cagggcacgg gcagcttgcc
      301 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
      361 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
      421 cccggtgaac agctcctcgc ccttgctcac catggcgcca gcgcaggcca tgcggccaca
      481 cactacccca gagtcggggg tcgggacaca gtgcacgccg tccaggccgc ggaagccgat
      541 gtacagcagg ttcccgttgc tggcgggcgg cggcgccacg gcgctcatct ccacactggc
      601 caggctgcag cgccgctgct tgtgctggca ggccgggcgc accttgtcct ccccgggcct
      661 cttcattcgc aggaaccacg cgcaccagtt cagaaggatg actctggtcc acttgggcat
      721 cttgcccccg tcggggtcgt ggtggtggta ctgcagcacg atcaccgtca ccaccaccga
      781 gaggcccacg atgatcatgg tgctggcgaa gtactgggct atcaatggta ccgaatcgga
      841 tgttgcgggc atgatctcag ccacgagcag catgaagacg gtaagagaga gtaagactgt
      901 tatccccagg gaaatcttct ccccggaatc tgcaggaagc aggaacacca gcagggcgag
      961 ggcggagatg agcacacagg ggatcagcag gttgaggcca tagtagagtg tcctgcggcg
     1021 catggtcact gtgaaggtga
//
