LOCUS       sequence-106770-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human Alpha7 (CHRNA7) with mEGFP fused into 
            M3-M4 loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Wang Y, Xiao C, Indersmitten T, Freedman R, Leonard S, Lester HA
  TITLE     The duplicated alpha7 subunits assemble and form functional 
            nicotinic receptors with the full-length alpha7.
  JOURNAL   J Biol Chem. 2014 Sep 19;289(38):26451-63. doi: 
            10.1074/jbc.M114.582858. Epub 2014 Jul 23.
  PUBMED    25056953
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.582858"; journalName: "J Biol Chem"; date: 
            "2014-09-19- 19"; volume: "289"; issue: "38"; pages: "26451-63"
COMMENT     Sequence Label: pcDNA3.1-CHRNA7-mGFP
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     166..187
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
       61 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      121 cacccagtcc aagcttagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      181 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg caggcgcctc
      241 ccccacgcac gatgagcacc tcctgcacgg tgggcaaccc cccgaggggg acccggactt
      301 ggccaagatc ctggaggagg tccgctacat tgccaaccgc ttccgctgcc aggacgaaag
      361 cgaggcggtc tgcagcgagt ggaagttcgc cgcctgtgtg gtggaccgcc tgtgcctcat
      421 ggccttctcg gtcttcacca tcatctgcac catcggcatc ctgatgtcgg ctcccaactt
      481 cgtggaggcc gtgtccaaag actttgcgta accacgcctg gttctgtaca tgtggaaaac
      541 tcacagatgg gcaaggcctt tggcttggcg agatttgggg gtgctaatcc aggacagcat
      601 tacacgccac aactccagtg ttcccttctg gctgtcagtc gtgttgctta cggtttcttt
      661 gttactttag gtagtagaat ctcagcactt tgtttcatat tctcagatgg gctgatagat
      721 atccttggca catccgtacc atcggtcagc agggccactg agtagtcatt ttgcccatta
      781 gcccactgcc tggaaagccc ttcggagagc tccccatggc tcctcaccac cgagacagtt
      841 ggttttgcat gtctgcatga aggtctacct gaaaattcaa catttgcttt tt
//
