LOCUS       sequence-106773-        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  t1 gRNA (ATGAGAATCAAGGCGGTCGA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Moore R, Spinhirne A, Lai MJ, Preisser S, Li Y, Kang T, Bleris L
  TITLE     CRISPR-based self-cleaving mechanism for controllable gene delivery 
            in human cells.
  JOURNAL   Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 
            10.1093/nar/gku1326. Epub 2014 Dec 18.
  PUBMED    25527740
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1326"; journalName: "Nucleic Acids Res"; date: 
            "2015-01-30- 30"; volume: "43"; issue: "2"; pages: "1297-303"
COMMENT     Sequence Label: pCas9–mKate2ps–T1gRNA
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    341..365
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(377..452)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(473..721)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(531..550)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(701..721)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     polyA_signal    814..861
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     886..905
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
       61 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      121 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga ggctatggca
      181 gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tggggggtgg
      241 ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg tgggatccac
      301 tagtaacggc cgccagtgtg ctggaattcg ccctttaatg ccaactttgt acaagaaagc
      361 tgggtctaga aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc
      421 ttattttaac ttgctatttc tagctctaaa actcgaccgc cttgattctc acggtgtttc
      481 gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt acggtaagca
      541 tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga aattattact
      601 ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta attctaatta
      661 tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga aataggccct
      721 cttcctgccc gaccttggta ccggatccag tcgactgagg tctcggtggg gtatcgacag
      781 agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca ccgtgcgttt
      841 tattctgtct ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg
      901 tttcagttag cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga
      961 ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg
     1021 cggtggaatc gaaatctcgt gatggcaggt tgggcgtc
//
