LOCUS       sequence-106774-        1103 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  t1 gRNA (ATGAGAATCAAGGCGGTCGA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Moore R, Spinhirne A, Lai MJ, Preisser S, Li Y, Kang T, Bleris L
  TITLE     CRISPR-based self-cleaving mechanism for controllable gene delivery 
            in human cells.
  JOURNAL   Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 
            10.1093/nar/gku1326. Epub 2014 Dec 18.
  PUBMED    25527740
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1326"; journalName: "Nucleic Acids Res"; date: 
            "2015-01-30- 30"; volume: "43"; issue: "2"; pages: "1297-303"
COMMENT     Sequence Label: pCas9–mKate2ps–T1gRNA
FEATURES             Location/Qualifiers
     source          1..1103
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(109..120)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ccttagtcag attatcgaac ttccgttgtg tgatcagttt ggcgttcagc agctgccgcc
       61 aataattttt cattttcttg acaacttctt ctgaggggac gttatcactc ttccctctat
      121 ttttatcgga tcttgtcaac actttattat caatagaatc atctttgaga aaagactggg
      181 gcacgatatg atccacgtcg tagtcggaga gccgattgat gtccagttcc tgatccacgt
      241 acatgtccct gccgttctgc aggtagtaca ggtagagctt ctcattctga agctgggtgt
      301 tttcaactgg gtgttcctta aggatttggg accccagttc ttttataccc tcttcaatcc
      361 tcttcatcct ttccctactg ttcttctgtc ccttctgggt agtttggttc tctcgggcca
      421 tctcgataac gatattctcg ggcttatgcc ttcccattac tttgacgagt tcatccacga
      481 ccttaacggt ctgcagtatt ccctttttga tagctgggct acctgcaaga ttagcgatgt
      541 gctcgtgaag actgtccccc tggccagaaa cttgtgcttt ctggatgtcc tccttaaagg
      601 tgagagagtc atcatggatc aactgcatga agttccggtt ggcaaatcca tcggacttaa
      661 gaaaatccag gattgtcttt ccactctgct tgtctcggat cccattgatc agttttcttg
      721 acagccgccc ccatcctgta tatcggcgcc tcttgagctg tttcatgact ttgtcgtcga
      781 agagatgagc gtaagttttc aagcgttctt caatcatctc cctatcttca aacaacgtaa
      841 gggtgaggac aatgtcctca agaatgtcct cgttctcctc attgtccagg aagtccttgt
      901 ctttaatgat tttcaggaga tcgtgatacg ttcccaggga tgcgttgaag cgatcctcca
      961 ctccgctgat ttcaacagag tcgaaacatt caatcttttt gaaatagtct tctttgagct
     1021 gtttcacggt aactttccgg ttcgtcttga agaggaggtc cacgatagct ttcttctgct
     1081 ctccagacag gaatgctggc ttt
//
