LOCUS       sequence-106776-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty; no U6 gRNA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Moore R, Spinhirne A, Lai MJ, Preisser S, Li Y, Kang T, Bleris L
  TITLE     CRISPR-based self-cleaving mechanism for controllable gene delivery 
            in human cells.
  JOURNAL   Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 
            10.1093/nar/gku1326. Epub 2014 Dec 18.
  PUBMED    25527740
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1326"; journalName: "Nucleic Acids Res"; date: 
            "2015-01-30- 30"; volume: "43"; issue: "2"; pages: "1297-303"
COMMENT     Sequence Label: pCas9–mKate2ps–EgRNA
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagccttgcc accatggaca
       61 agaagtactc cattgggctc gatatcggca caaacagcgt cggctgggcc gtcattacgg
      121 acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat cgccacagca
      181 taaagaagaa cctcattggc gccctcctgt tcgactccgg ggagacggcc gaagccacgc
      241 ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc tgctacctgc
      301 aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat aggctggagg
      361 agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt ggcaatatcg
      421 tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg aagaagcttg
      481 tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg catatgatca
      541 aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc gatgtcgaca
      601 aactctttat ccaactggtt cagacttaca atcagctttt cgaagagaac ccgatcaacg
      661 catccggagt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc cggcggctcg
      721 aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt aatcttatcg
      781 ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc gaagatgcca
      841 agcttcaact gagcaaagac acctacgatg atgatctcga caatctgctg gcccagatcg
      901 gcgaccagta cgcagacctt tttttggcgg caaagaacct gtcagacgcc attctgctga
      961 gtgatattct gcgagtgaac acggagatca ccaaagctcc gctgagcgct agtatgatca
     1021 agcgctatga tgagcaccac caag
//
