LOCUS       sequence-106777-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty; no U6 gRNA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Moore R, Spinhirne A, Lai MJ, Preisser S, Li Y, Kang T, Bleris L
  TITLE     CRISPR-based self-cleaving mechanism for controllable gene delivery 
            in human cells.
  JOURNAL   Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 
            10.1093/nar/gku1326. Epub 2014 Dec 18.
  PUBMED    25527740
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1326"; journalName: "Nucleic Acids Res"; date: 
            "2015-01-30- 30"; volume: "43"; issue: "2"; pages: "1297-303"
COMMENT     Sequence Label: pCas9–mKate2ps–EgRNA
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(157..276)
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             complement(295..990)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatct agagtcgcgg ccgctggccg gccttagacg ttgatcctgg cgctggcgca
      181 agcagcaggg tgtctatcca tgccgctctc ctgggcgcag ctcatgggca gggtgccggc
      241 ggcctgctcc tccacctcgg gagggaagcc gtgagatccg gaacctcctc cacctctgtg
      301 ccccagtttg ctagggaggt cgcagtatct ggccacagcc acctcgtgct gctcgacgta
      361 ggtctctttg tcggcctcct tgattctttc cagtcttctg tccacatagt agacgccggg
      421 catcttgagg ttcttagcgg gtttcttgga tctgtatgtg gtcttcaagt tgcagatcag
      481 gtggcccccg cccacgagct tcagggccat gtcggctctg ccttccaggc cgccgtcagc
      541 ggggtacagg gtctcggtgg aggcctccca gccgagtgtt ttcttctgca tcacagggcc
      601 gttggatggg aagttcaccc ctctgatctt gacgttgtag atgaggcagc cgtcctggag
      661 gctggtgtcc tgggtagcgg tcagcacgcc cccgtcttcg tatgtggtga ctctctccca
      721 tgtgaagccc tcggggaagg actgcttaaa gaagtcgggg atgccctggg tgtggttgat
      781 gaaggttttg ctgccgtaca tgaagctggt agccaggatg tcgaaggcga aggggagagg
      841 gccgccctcg accgccttga ttctcatggt ctgggtgccc tcgtagggct tgccttcgcc
      901 ctcggatgtg cacttgaagt ggtggttgtt cacggtgccc tccatgtaca gcttcatgtg
      961 catgttctcc ttaatcagct cgctcaccat gggccctccg gaa
//
