LOCUS       sequence-106778-        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty; no U6 gRNA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Moore R, Spinhirne A, Lai MJ, Preisser S, Li Y, Kang T, Bleris L
  TITLE     CRISPR-based self-cleaving mechanism for controllable gene delivery 
            in human cells.
  JOURNAL   Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 
            10.1093/nar/gku1326. Epub 2014 Dec 18.
  PUBMED    25527740
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1326"; journalName: "Nucleic Acids Res"; date: 
            "2015-01-30- 30"; volume: "43"; issue: "2"; pages: "1297-303"
COMMENT     Sequence Label: pCas9–mKate2ps–EgRNA
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    340..387
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     412..431
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     primer_bind     complement(730..749)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
       61 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
      121 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg
      181 cagggcctgc cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt
      241 ggggtgggga aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat
      301 cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt
      361 gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct
      421 tccgtgtttc agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc
      481 gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga
      541 aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt
      601 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
      661 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
      721 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
      781 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
      841 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc
      901 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
      961 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     1021 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     1081 ctcggcagga g
//
