LOCUS       sequence-106779-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty; no U6 gRNA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Moore R, Spinhirne A, Lai MJ, Preisser S, Li Y, Kang T, Bleris L
  TITLE     CRISPR-based self-cleaving mechanism for controllable gene delivery 
            in human cells.
  JOURNAL   Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 
            10.1093/nar/gku1326. Epub 2014 Dec 18.
  PUBMED    25527740
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1326"; journalName: "Nucleic Acids Res"; date: 
            "2015-01-30- 30"; volume: "43"; issue: "2"; pages: "1297-303"
COMMENT     Sequence Label: pCas9–mKate2ps–EgRNA
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(107..118)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttagtcagat tatcgaactt ccgttgtgtg atcagtttgg cgttcagcag ctgccgccaa
       61 taatttttca ttttcttgac aacttcttct gaggggacgt tatcactctt ccctctattt
      121 ttatcggatc ttgtcaacac tttattatca atagaatcat ctttgagaaa agactggggc
      181 acgatatgat ccacgtcgta gtcggagagc cgattgatgt ccagttcctg atccacgtac
      241 atgtccctgc cgttctgcag gtagtacagg tagagcttct cattctgaag ctgggtgttt
      301 tcaactgggt gttccttaag gatttgggac cccagttctt ttataccctc ttcaatcctc
      361 ttcatccttt ccctactgtt cttctgtccc ttctgggtag tttggttctc tcgggccatc
      421 tcgataacga tattctcggg cttatgcctt cccattactt tgacgagttc atccacgacc
      481 ttaacggtct gcagtattcc ctttttgata gctgggctac ctgcaagatt agcgatgtgc
      541 tcgtgaagac tgtccccctg gccagaaact tgtgctttct ggatgtcctc cttaaaggtg
      601 agagagtcat catggatcaa ctgcatgaag ttccggttgg caaatccatc ggacttaaga
      661 aaatccagga ttgtctttcc actctgcttg tctcggatcc cattgatcag ttttcttgac
      721 agccgccccc atcctgtata tcggcgcctc ttgagctgtt tcatgacttt gtcgtcgaag
      781 agatgagcgt aagttttcaa gcgttcttca atcatctccc tatcttcaaa caacgtaagg
      841 gtgaggacaa tgtcctcaag aatgtcctcg ttctcctcat tgtccaggaa gtccttgtct
      901 ttaatgattt tcaggagatc gtgatacgtt cccagggatg cgttgaagcg atcctccact
      961 ccgctgattt caacagagtc gaaacattca atctttt
//
