LOCUS       sequence-106780-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty; no U6 gRNA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Moore R, Spinhirne A, Lai MJ, Preisser S, Li Y, Kang T, Bleris L
  TITLE     CRISPR-based self-cleaving mechanism for controllable gene delivery 
            in human cells.
  JOURNAL   Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 
            10.1093/nar/gku1326. Epub 2014 Dec 18.
  PUBMED    25527740
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1326"; journalName: "Nucleic Acids Res"; date: 
            "2015-01-30- 30"; volume: "43"; issue: "2"; pages: "1297-303"
COMMENT     Sequence Label: pCas9–mKate2ps–EgRNA
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(51..104)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(108..128)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttcatgtgca tgttctcctt aatcagctcg ctcaccatgg gccctccgga agggccggga
       61 ttctcctcca cgtcaccgca tgttagaaga cttcctctgc cctcagccac cttcctcttc
      121 ttcttggggt cagccctgct gtctccaccg agctgagaga ggtcgattct tgtttcatag
      181 agccccgtaa ttgactgatg aatcagtgtg gcgtccagga cctcctttgt agaggtgtac
      241 cgctttctgt ctatggtggt gtcgaagtac ttgaaggctg caggcgcgcc caagttggtc
      301 agagtaaaca agtggataat gttttctgcc tgctccctga tgggcttatc cctgtgctta
      361 ttgtaagcag aaagcacctt atcgaggtta gcgtcggcga ggatcactct tttggagaat
      421 tcgcttattt gctcgatgat ctcatcaagg tagtgtttgt gttgttccac gaacagctgc
      481 ttctgctcat tatcttcggg agaccctttg agcttttcat agtggctggc cagatacaag
      541 aaattaacgt atttagaggg cagtgccagc tcgttacctt tctgcagctc gcccgcacta
      601 gcgagcattc gtttccggcc gttttcaagc tcaaagagag agtacttggg aagcttaatg
      661 atgaggtctt ttttgacctc tttatatcct ttcgcctcga gaaagtcgat ggggtttttt
      721 tcgaagcttg atcgctccat gattgtgatg cccagcagtt ccttgacgct tttgagtttt
      781 ttagacttcc ctttctccac tttggccaca accagtacac tgtaagcgac tgtaggagaa
      841 tcgaatccgc cgtatttctt ggg
//
