LOCUS       sequence-106795-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEGFP-H4-23
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(138..851,852..854,855..857))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(183..204)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     532..551
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     791..812
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      854..863
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agctgcataa cagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
       61 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
      121 ctagagtcgc ggccgcttta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
      181 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg
      241 gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
      301 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
      361 aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg
      421 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
      481 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
      541 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac
      601 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag
      661 gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac
      721 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
      781 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
      841 tcgcccttgc tcaccatggt ggcgaccggt ggatcccggg cccgcggtac cgtcga
//
