LOCUS       sequence-106800-         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RPL16b HA-TEV-AVI pop-in/pop-out plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Jan CH, Williams CC, Weissman JS
  TITLE     Principles of ER cotranslational translocation revealed by 
            proximity-specific ribosome profiling.
  JOURNAL   Science. 2014 Nov 7;346(6210):1257521. doi: 10.1126/science.1257521.
            Epub 2014 Nov 6.
  PUBMED    25378630
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2014-11-7"; volume: "346(6210):1257521. doi"; pages: " 
            10.1126/science.1257521. Epub 2014 Nov 6"
COMMENT     Sequence Label: pJW1506
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     47..63
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     48..64
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             475..501
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             511..531
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             535..579
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(898..917)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(899..917)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga ggtcgacggt
       61 atcgataagc ttgatatcga attcctgcag cccgggggat ccactagtct ttacaaggct
      121 attcgtggta tggtttccca caagaccgcc cgtggtaagg ctgccttgga acgtttaaag
      181 atcttcgaag gtattccacc tccatacgac aaaaagaaga gagttgtcgt tcctcaagct
      241 ttgagagttt tgagattgaa accaggtaga aagtacacca ctttgggtaa gttgtctact
      301 tctgttggtt ggaaatacga agatgttgtt gctaaattag aagacaagag aaaggtcaga
      361 tccgccgaat attacgccaa gaagagagct ttcaccaaaa aggtctcctc tgcttctgct
      421 gctgcttctg aatccgatgt tgctaagcaa ttggcttctt tcggttacgg ttcttaccca
      481 tacgacgtcc cagactacgc tggaggttca gaaaatttgt attttcaagg atcaggtttg
      541 aatgatattt ttgaagctca aaaaattgaa tggcatgagt aaatttcatg aaatgaattt
      601 gtgatcaatc ttatctttta ttttttgcaa aacaacaaac caccctacat ttttagttat
      661 ttttatttat tttgtttcac attttaaata atatcaataa cagataaata aacatattaa
      721 aacactggta taaattctaa ttgatcagat tttttgaata tgaaccacat cttaaagaaa
      781 ggtacctaaa ggacattcta gagacttcga attttcacca tatcaaaaca aagcgtgaaa
      841 gaataacaat atgcactagt tctagagcgg ccgccaccgc ggtggagctc caattcgccc
      901 tatagtgagt cgtattacaa ttcactggcc gtcgtt
//
