LOCUS       sequence-106806-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCR2.1 linker-tev-linker-3X-FLAG::KanMX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Williams CC, Jan CH, Weissman JS
  TITLE     Targeting and plasticity of mitochondrial proteins revealed by 
            proximity-specific ribosome profiling.
  JOURNAL   Science. 2014 Nov 7;346(6210):748-51. doi: 10.1126/science.1257522.
  PUBMED    25378625
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-11-7- 7"; volume: "346"; issue: 
            "6210"; pages: "748-51"
COMMENT     Sequence Label: pJW1514
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(38..381)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      complement(506..753)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     719..737
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     755..771
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     756..772
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(778..849)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             complement(862..882)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atcgcggcct cgaaacgtga gtcttttcct tacccatggt tgtttatgtt cggatgtgat
       61 gtgagaactg tatcctagca agattttaaa aggaagtata tgaaagaaga acctcagtgg
      121 caaatcctaa ccttttatat ttctctacag gggcgcggcg tggggacaat tcaacgcgtc
      181 tgtgagggga gcgtttccct gctcgcaggt ctgcagcgag gagccgtaat ttttgcttcg
      241 cgccgtgcgg ccatcaaaat gtatggatgc aaatgattat acatggggat gtatgggcta
      301 aatgtacggg cgacagtcac atcatgcccc tgagctgcgc acgtcaagac tgtcaaggag
      361 ggtattctgg gcctccatgt cgctggccgg gtgacccggc ggggacgagg caagctaaac
      421 agatctggcg cgccttaatt aacccgggga tccgtcgacc tgcagcgtac gaagcttcag
      481 ctggcggccg cgttcggtac cggccgcaaa ttaaagcctt cgagcgtccc aaaaccttct
      541 caagcaaggt tttcagtata atgttacatg cgtacacgcg tctgtacaga aaaaaaagaa
      601 aaatttgaaa tataaataac gttcttaata ctaacataac tataaaaaaa taaataggga
      661 cctagacttc aggttgtcta actccttcct tttcggttag agcggatgtg gggggagggc
      721 gtgaatgtaa gcgtgacata actaattaca tgactcgagg tcgacggtat cgatttactt
      781 gtcatcgtca tctttataat ccttgtcatc gtcatcttta taatccttgt catcgtcatc
      841 tttataatca gaacctgaac cagattgaaa atacaaattt tcacctgaac ctgaacctcc
      901 aagggcgaat tctgcagata tccatcacac tggcggccgc tcgagcatgc atctagagg
//
