LOCUS       sequence-106807-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of EGFP-TEV-S-tag CENP-K for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pIC144
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..67
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             80..124
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gtccggaggg gatcggtgga gctccaccgg tggcggccgc tctagagaga atctttattt
       61 tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga
      121 cagcggaggt ggaggtacta gtctcgagat gaatcaggag gatttagatc cggatagtac
      181 tacagatgtg ggagatgtta caaatactga agaagaactt attagagaat gtgaagaaat
      241 gtggaaagat atggaagaat gtcagaataa attatcactt attggaactg aaacactcac
      301 cgattcaaat gctcagctat cattgttaat tatgcaagta aaatgtttaa ccgctgaact
      361 cagtcaatgg cagaaaaaaa cacctgaaac aattcccttg actgaagacg ttctcataac
      421 attaggaaaa gaagagttcc aaaagctgag acaagatctt gaaatggtac tgtccactaa
      481 ggagtcaaag aatgaaaagt taaaggaaga cttagaaagg gaacaacggt ggttggatga
      541 acagcaacag ataatggaat ctcttaatgt actacacagt gaattgaaaa ataaggttga
      601 aacattttct gaatcaagaa tctttaatga actgaaaact aaaatgctta atataaaaga
      661 atataaggag aaactcttga gtaccttggg cgagtttcta gaagaccatt ttcctctgcc
      721 tgatagaagt g
//
