LOCUS       sequence-10681-e        1548 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-NLK
FEATURES             Location/Qualifiers
     source          1..1548
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..66
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 atggcggctt acaatggcgg tacatctgca gcagcaacag gtcaccacca ccaccatcac
       61 caccaccttc cacacctccc tcctcctcac ctgcaccacc accaccaccc tcaacaccat
      121 cttcatccgg ggtcggctgc cgctgtacac cctgtacagc agcacacctc ttcggcagct
      181 gcggcagccg cagcagcggc tgcagctgca gccatgttaa accctgggca acaacagcca
      241 tatttcccat caccggcacc ggggcaggct cctggaccag ctgcagcagc cccagctcag
      301 gtacaggctg ccgcagctgc tacagttaag gcgcaccatc atcagcactc gcatcatcca
      361 cagcagcagc tggatattga gccggataga cctattggat atggagcctt tggtgttgtc
      421 tggtcagtaa cagatccaag agatggaaag agagtagcgc tcaaaaagat gcccaacgtc
      481 ttccagaatc tggtctcttg caaaagggtc ttccgggaat tgaagatgtt gtgttttttt
      541 aagcatgata atgtactctc tgcccttgac atactccaac ctccacacat tgactatttt
      601 gaagaaatat atgttgtcac agaattgatg cagagtgacc tacataaaat tatcgtctct
      661 cctcaaccac tcagctcaga tcatgtcaaa gtttttcttt atcagatttt gcgaggtttg
      721 aaatatctcc attcagctgg cattttacat cgagacatta agccagggaa tctccttgtg
      781 aacagcaact gtgttctaaa gatttgtgat tttggattgg ccagagtgga agaattagat
      841 gaatcccgtc atatgactca ggaagttgtt actcagtatt atcgggctcc agaaatcctg
      901 atgggcagcc gtcattacag caatgctatt gacatctggt ctgtgggatg tatctttgca
      961 gaactactag gacgaagaat attgtttcag gcacagagtc ccattcagca gttggatttg
     1021 atcacggatc tgttgggcac accatcactg gaagcaatga ggacagcttg tgaaggcgct
     1081 aaggcacata tactcagggg tcctcataaa cagccatctc ttcctgtact ctataccctg
     1141 tctagccagg ctacacatga agctgttcat ctcctttgca ggatgttggt ctttgatcca
     1201 tccaaaagaa tatccgctaa ggatgcctta gcccacccct acctagatga agggcgacta
     1261 cgatatcaca catgtatgtg taaatgttgc ttttccacct ccactggaag agtttatacc
     1321 agtgactttg agcctgtcac caatcccaaa tttgatgaca ctttcgagaa gaacctcagt
     1381 tctgtccgac aggttaaaga aattattcat cagttcattt tggaacagca gaaaggaaac
     1441 agagtgcctc tctgcatcaa ccctcagtct gctgctttta agagctttat tagttccact
     1501 gttgctcagc catctgagat gcccccatct cctctggtgt gggagtga
//
