LOCUS       sequence-106811-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS305 pPGK1-BirA::LEU2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Jan CH, Williams CC, Weissman JS
  TITLE     Principles of ER cotranslational translocation revealed by 
            proximity-specific ribosome profiling.
  JOURNAL   Science. 2014 Nov 7;346(6210):1257521. doi: 10.1126/science.1257521.
            Epub 2014 Nov 6.
  PUBMED    25378630
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2014-11-7"; volume: "346(6210):1257521. doi"; pages: " 
            10.1126/science.1257521. Epub 2014 Nov 6"
COMMENT     Sequence Label: pJW1234
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(64..311)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     277..295
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga ggtcgacaag
       61 cttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt tcagtataat
      121 gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata taaataacgt
      181 tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag gttgtctaac
      241 tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc gtgacataac
      301 taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt ttttcaagta
      361 ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca tcttggtacc
      421 gggccccccc tcgactagtg attgccatgg tcattatttt tctgcactac gcagggatat
      481 ttcaccgccc atccagggtt ttattattcc atcctgctca agtaataaag ccccctgttt
      541 gtctattccg cgtgaaatgc caaatatttc tttatcacca atgataagtt tcactgggcg
      601 attaataaaa ttatccagct tttcccagcg cgacagataa ggtgccaatc cttcttgttc
      661 gaagagttcc aacgcagcac gtaattcacg tattagcatg gccgccaacg tattacgatc
      721 gagattgatc cccgcttcct gcagcgtgat ccacccctga ttaacgacac tctcttcaac
      781 acggcgcatt gccatgttga tcccggctcc aatgactatt tgcgccgcat cgccagtt
//
