LOCUS       sequence-106813-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of EGFP-TEV-S-tag FANC-A for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKG186
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..67
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             80..124
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gtccggaggg gatcggtgga gctccaccgg tggcggccgc tctagagaga atctttattt
       61 tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga
      121 cagcggaggt ggaggtacta gtctcgagat gtccgactcg tgggtcccga actccgcctc
      181 gggccaggac ccagggggcc gccggagggc ctgggccgag ctgctggcgg gaagggtcaa
      241 gagggaaaaa tataatcctg aacgggcaca gaaattaaag gaatcagctg tgcgcctcct
      301 gcgaagccat caggacctga atgccctttt gcttgaggta gaaggtccac tgtgtaaaaa
      361 attgtctctc agcaaagtga ttgactgtga cagttctgag gcctatgcta atcattctag
      421 ttcatttata ggctctgctt tgcaggatca agcctcaagg ctgggggttc ccgtgggtat
      481 tctctcagcc gggatggttg cctctagcgt gggacagatc tgcacggctc cagcggagac
      541 cagtcaccct gtgctgctga ctgtggagca gagaaagaag ctgtcttccc tgttagagtt
      601 tgctcagtat ttattggcac acagtatgtt ctcccgtctt tccttctgtc aagaattatg
      661 gaaaatacag agttctttgt tgcttgaagc ggtgtggcat cttcacgtac aaggcattgt
      721 gagcctgcaa gagctgctgg
//
