LOCUS       sequence-106817-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of EGFP-TEV-S-tag CENP-W for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKG136
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..67
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             80..124
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(591..611)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtccggaggg gatcggtgga gctccaccgg tggcggccgc tctagagaga atctttattt
       61 tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga
      121 cagcggaggt ggaggtacta gtctcgagat ggcactgtct acgattgtga gtcagcgtaa
      181 acaaatcaaa cgtaaagccc ctcgtggttt cctgaaacgt gtgtttaaac gtaaaaaacc
      241 tcagctgcgc ctggaaaaat caggtgatct gctggttcat ctgaattgcc tgttattcgt
      301 ccaccgtctg gcagaagaat cccgtactaa tgcctgcgct tcgaaatgtc gtgtcatcaa
      361 caaagaacat gtgctggctg ccgctaaagt cattctgaaa aaatcccgtg gctgagaatt
      421 ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca
      481 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
      541 acctgaaaca taaaatgaat gcaattcgcc agcacagtgg tcgaccctgt ggaatgtgtg
      601 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
      661 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
      721 gcaaagcatg catctcaatt agtcagcaac catagt
//
