LOCUS       sequence-106819-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of EGFP-TEV-S-tag CENP-W for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKG136
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(439..483)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(496..516)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(604..625)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
       61 acctctacaa atgtggtatg gctgattatg atcagttatc tagatccggt ggatcccggg
      121 cccgcggtac cgtcgactgc agaattctca gccacgggat tttttcagaa tgactttagc
      181 ggcagccagc acatgttctt tgttgatgac acgacatttc gaagcgcagg cattagtacg
      241 ggattcttct gccagacggt ggacgaataa caggcaattc agatgaacca gcagatcacc
      301 tgatttttcc aggcgcagct gaggtttttt acgtttaaac acacgtttca ggaaaccacg
      361 aggggcttta cgtttgattt gtttacgctg actcacaatc gtagacagtg ccatctcgag
      421 actagtacct ccacctccgc tgtccatgtg ctggcgttcg aatttagcag cagcggtttc
      481 tttgaatttg gcggcgccct gaaaataaag attctctcta gagcggccgc caccggtgga
      541 gctccaccga tcccctccgg acttgtacag ctcgtccatg ccgagagtga tcccggcggc
      601 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
      661 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
      721 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
      781 g
//
