LOCUS       sequence-106833-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Ramaswamy S, Nakamura N, Vazquez F, Batt DB, Perera S, Roberts TM, 
            Sellers WR
  TITLE     Regulation of G1 progression by the PTEN tumor suppressor protein is
            linked to inhibition of the phosphatidylinositol 3-kinase/Akt 
            pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 1999 Mar 2. 96(5):2110-5.
  PUBMED    10051603
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1999 Mar 2. 96(5):2110-5."
COMMENT     Sequence Label: 1013 pcDNA3 T7 Akt1 T308A S473A
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(705..723)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(706..723)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(743..760)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    749..860
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggtgtaccgg gacctcaagc tggagaacct catgctggac aaggacgggc acattaagat
       61 cacagacttc gggctgtgca aggaggggat caaggacggt gccaccatga aggccttttg
      121 cggcacacct gagtacctgg cccccgaggt gctggaggac aatgactacg gccgtgcagt
      181 ggactggtgg gggctgggcg tggtcatgta cgagatgatg tgcggtcgcc tgcccttcta
      241 caaccaggac catgagaagc tttttgagct catcctcatg gaggagatcc gcttcccgcg
      301 cacgcttggt cccgaggcca agtccttgct ttcagggctg ctcaagaagg accccaagca
      361 gaggcttggc gggggctccg aggacgccaa ggagatcatg cagcatcgct tctttgccgg
      421 tatcgtgtgg cagcacgtgt acgagaagaa gctcagccca cccttcaagc cccaggtcac
      481 gtcggagact gacaccaggt attttgatga ggagttcacg gcccagatga tcaccatcac
      541 accacctgac caagatgaca gcatggagtg tgtggacagc gagcgcaggc cccacttccc
      601 ccagttcgcc tactcggcca gcggcacggc ctgaggcggc ggtggaattc tgcagatatc
      661 catcacactg gcggccgctc gagcatgcat ctagagggcc ctattctata gtgtcaccta
      721 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
      781 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
      841 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
      901 gtggggcagg acagcaaggg ggaggattgg gaa
//
