LOCUS       sequence-106836-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation Lentiviral vector for bi-cistronic expression of EGFP
            and the gene of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Lou E, Fujisawa S, Morozov A, Barlas A, Romin Y, Dogan Y, Gholami S,
            Moreira AL, Manova-Todorova K, Moore MA
  TITLE     Tunneling nanotubes provide a unique conduit for intercellular 
            transfer of cellular contents in human malignant pleural 
            mesothelioma.
  JOURNAL   PLoS One. 2012;7(3):e33093. doi: 10.1371/journal.pone.0033093. Epub 
            2012 Mar 9.
  PUBMED    22427958
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3):e33093. doi"; pages: " 
            10.1371/journal.pone.0033093. Epub 2012 Mar 9"
COMMENT     Sequence Label: pUltra
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             90..323
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             520..531
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tttnngcgca taacagttcc tctcactctc tgatattcat ttctttgcaa gttataaata
       61 ctgaataata agatgacatg aactactact gctagagatt ttccacactg actaaaaggg
      121 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac
      181 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
      241 ggctcagatc tggtctaacc agagagaccc agtacaagca aaaagcagat cttgtcttcg
      301 ttgggagtta cgtagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
      361 agctgccttg taagtcattg gtcttaaagg taccgagctc gaattaattc caggcgggga
      421 ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagatgcg gaagaggccg
      481 cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg aagggacgta
      541 gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc atggaaagga
      601 cgtcagcttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag cccctgtcca
      661 gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg ggaaagcgaa
      721 agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc
      781 aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct cataaagaga
      841 cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag caatagcatg
//
