LOCUS       sequence-106838-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of BRAF V600E (the V600E mutation has been 
            found in cancer patients, confers increased kinase activity, and is 
            transforming).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene.
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pBabe-Puro-BRAF-V600E
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..21)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(37..66)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctcatggtga tggtgatgat gaccggtatg catattcaga tcctcttctg agatgagttt
       61 ttgttcgaag ggccctctag actcgaaaac gaattcgccc tttccgtgga caggaaacgc
      121 accatatccc cctgcctgga tgggtgtttt tggagaagca caagcatata gactaaaatc
      181 ctctgtttgg aaaccagccc gattcaagga gggttctgat gcactgcggt gaatttttgg
      241 caatgagcgg gccagcagct caatagaggc gagaatttgg ggaaagagtg gtctctcatc
      301 tcttttcttt ttgaggcact ctgccattaa tctcttcatg gcttttggac agttactccg
      361 taccttactg agatctggag acaggtatcc tcgtcccacc ataaaaatta tctggtccct
      421 gttgttgatg tttgaataag gtaactgtcc agtcatcaat tcatacagaa caattccaaa
      481 tgcatataca tctgactgaa agctgtatgg atttttatct tgcattctga tgacttctgg
      541 tgccatccac aaaatggatc cagacaactg ttcaaactga tgggacccac tccatcgaga
      601 tttctctgta gctagaccaa aatcacctat ttttactgtg aggtcttcat gaagaaatat
      661 attattactc ttgaggtctc tgtggatgat tgacttggcg tgtaagtaat ccatgccctg
      721 tgcagtctgt cgtgcaatat ctataagttt gatcatctca aatttggtct caatgatatg
      781 gagatggtga tacaagctgg agccctcaca ccactgggta acaatagcca gttgtggctt
      841 tgtggaatag cccatgaaga gtaggatatt caca
//
