LOCUS       sequence-106839-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of BRAF V600E (the V600E mutation has been 
            found in cancer patients, confers increased kinase activity, and is 
            transforming).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene.
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pBabe-Puro-BRAF-V600E
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(29..45)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(30..46)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gagttaacga attctacatc aagcttatcg ataccgtcga cctcgaatgg cggcgctgag
       61 cggtggcggt ggtggcggcg cggagccggg ccaggctctg ttcaacgggg acatggagcc
      121 cgaggccggc gccggcgccg gcgccgcggc ctcttcggct gcggaccctg ccattccgga
      181 ggaggtgtgg aatatcaaac aaatgattaa gttgacacag gaacatatag aggccctatt
      241 ggacaaattt ggtggggagc ataatccacc atcaatatat ctggaggcct atgaagaata
      301 caccagcaag ctagatgcac tccaacaaag agaacaacag ttattggaat ctctggggaa
      361 cggaactgat ttttctgttt ctagctctgc atcaatggat accgttacat cttcttcctc
      421 ttctagcctt tcagtgctac cttcatctct ttcagttttt caaaatccca cagatgtggc
      481 acggagcaac cccaagtcac cacaaaaacc tatcgttaga gtcttcctgc ccaacaaaca
      541 gaggacagtg gtacctgcaa ggtgtggagt tacagtccga gacagtctaa agaaagcact
      601 gatgatgaga ggtctaatcc cagagtgctg tgctgtttac agaattcagg atggagagaa
      661 gaaaccaatt ggttgggaca ctgatatttc ctggcttact ggagaagaat tgcatgtgga
      721 agtgttggag aatgttccac ttacaacaca caactttgta cgaaaaacgt ttttcacctt
      781 agcattttgt gacttttgtc gaaagctgct tttccagggt ttccgctgtc aaacatgtgg
      841 ttataaattt caccagcgtt gtagtacaga agttccactg atgtgtgtta attatgacca
      901 acttgatttg ctgtttgtct ccaagttctt tgaacaccac ccaataccac aggaagagg
//
