LOCUS       sequence-106840-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway compatible Y2H vector - BD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Lu Q, Tang X, Tian G, Wang F, Liu K, Nguyen V, Kohalmi SE, Keller 
            WA, Tsang EW, Harada JJ, Rothstein SJ, Cui Y
  TITLE     Arabidopsis homolog of the yeast TREX-2 mRNA export complex: 
            components and anchoring nucleoporin.
  JOURNAL   Plant J. 2010 Jan;61(2):259-70. doi: 
            10.1111/j.1365-313X.2009.04048.x. Epub 2009 Oct 16.
  PUBMED    19843313
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-313X.2009.04048.x"; journalName: "Plant J"; date: 
            "2010-01"; volume: "61"; issue: "2"; pages: "259-70"
COMMENT     Sequence Label: pGBKT7-GW
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             161..601
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     612..631
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        612..630
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             650..679
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    686..810
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gctgtcttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat
       61 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag
      121 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat
      181 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa
      241 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc
      301 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct
      361 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca
      421 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt
      481 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat
      541 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc
      601 gccggaattt gtaatacgac tcactatagg gcgagccgcc atcatggagg agcagaagct
      661 gatctcagag gaggacctgc atatgacaag tttgtacaaa aaagctgaac gagaaacgta
      721 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
      781 ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcaccc
//
