LOCUS       sequence-106843-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway compatible BiFC vector (pEarleyGate201-YN).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Lu Q, Tang X, Tian G, Wang F, Liu K, Nguyen V, Kohalmi SE, Keller 
            WA, Tsang EW, Harada JJ, Rothstein SJ, Cui Y
  TITLE     Arabidopsis homolog of the yeast TREX-2 mRNA export complex: 
            components and anchoring nucleoporin.
  JOURNAL   Plant J. 2010 Jan;61(2):259-70. doi: 
            10.1111/j.1365-313X.2009.04048.x. Epub 2009 Oct 16.
  PUBMED    19843313
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-313X.2009.04048.x"; journalName: "Plant J"; date: 
            "2010-01"; volume: "61"; issue: "2"; pages: "259-70"
COMMENT     Sequence Label: pGTQL1211YN
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    31..155
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(196..501)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRIVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(298..317)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ttgctcacca ttgtagcgat actcctaggc accactttgt acaagaaagc tgaacgagaa
       61 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
      121 taatactgta aaacacaaca tatccagtca ctatggtcga cctgcagact ggctgtgtat
      181 aagggagcct gacatttata ttccccagaa catcaggtta atggcgtttt tgatgtcatt
      241 ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gagaccggca cactggccat
      301 atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt aaagttcacg
      361 ggagacttta tctgacagca gacgtgcact ggccaggggg atcactatcc gtcgcccggg
      421 cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct ctcttttata
      481 ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc gtcagcaaaa gagccgttca
      541 tttcaataaa ccgggcgacc tcagccatcc cttcctgatt ttccgctttc cagcgttcgg
      601 cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cagaccggag atattgacat
      661 catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa tacgctgctt
      721 catagcatac ctctttttga catacttcgg gtatacatat cagtatatat tcttataccg
      781 caaa
//
