LOCUS       sequence-106845-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and 
            cloning backbone for sgRNA (V2.0).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F
  TITLE     Genome engineering using the CRISPR-Cas9 system.
  JOURNAL   Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. 
            Epub 2013 Oct 24.
  PUBMED    24157548
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.143"; journalName: "Nat Protoc"; date: 
            "2013-11"; volume: "8"; issue: "11"; pages: "2281-308"
COMMENT     Sequence Label: pSpCas9n(BB)-2A-Puro (PX462) V2.0
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccagttcgct caggccgcct ctctcggcct tggtcagatt gtcgaacttt ctctgggtaa
       61 tcagcttggc gttcagcagc tgccgccagt agttcttcat cttcttcacg acctcttcgg
      121 agggcacgtt gtcgctcttg ccccggttct tgtcgcttct ggtcagcacc ttgttgtcga
      181 tggagtcgtc cttcagaaag ctctgaggca cgatatggtc cacatcgtag tcggacagcc
      241 ggttgatgtc cagttcctgg tccacgtaca tatcccgccc attctgcagg tagtacaggt
      301 acagcttctc gttctgcagc tgggtgtttt ccacggggtg ttctttcagg atctggctgc
      361 ccagctcttt gatgccctct tcgatccgct tcattctctc gcggctgttc ttctgtccct
      421 tctgggtggt ctggttctct ctggccattt cgatcacgat gttctcgggc ttgtgccggc
      481 ccatcacttt cacgagctcg tccaccacct tcactgtctg caggatgccc ttcttaatgg
      541 cggggctgcc ggccagattg gcaatgtgct cgtgcaggct atcgccctgg ccggacacct
      601 gggctttctg gatgtcctct ttaaaggtca ggctgtcgtc gtggatcagc tgcatgaagt
      661 ttctgttggc gaagccgtcg gacttcagga aatccaggat tgtcttgccg gactgcttgt
      721 cccggatgcc gttgatcagc ttccggctca gcctgcccca gccggtgtat ctccgccgct
      781 tcagctgctt catcactttg tcgtcgaaca ggtgggcata ggttttcagc cgttcctcga
      841 tcatctctct gtcctcaaac agtgtcaggg tcagcacgat atcttccaga atgtcctcgt
      901 tttcctcatt gtccaggaag tccttgtcct tgataatttt cagcagatcg tggtatgtgc
      961 ccagggaggc gttgaaccga tcttccacgc cggagatttc cacggagtcg aagcactcga
     1021 ttttcttgaa gtagtcctct tt
//
