LOCUS       sequence-106848-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  reporter plasmid that encodes one of five synthetic 
            sgRNA-repressible promoters that express mRFP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Nielsen AA, Voigt CA
  TITLE     Multi-input CRISPR/Cas genetic circuits that interface host 
            regulatory networks.
  JOURNAL   Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
  PUBMED    25422271
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-24"; volume: "10:763. doi"; pages: " 
            10.15252/msb.20145735"
COMMENT     Sequence Label: pAN-PA1-RFP
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        258..292
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     misc_RNA        295..344
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
ORIGIN
        1 cgcttggcct cgcgcacaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
       61 accaaggtag tcggcaaata atactagctc cggcaaaaaa acgggcaagg tgtcaccacc
      121 ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga cgtctaagaa
      181 ctcggtacca aaccaattat tgaagacgct gaaaagcgtc tttttttgtt tcggtcctcc
      241 gaatgacatg cgtctccttt acacctagct cagtcctagg tattatgcta gcagctgtca
      301 ccggatgtgc tttccggtct gatgagtccg tgaggacgaa acagcctcta caaataattt
      361 tgtttaatac tagagtcaca caggaaagta ctagatggct tcctccgaag acgttatcaa
      421 agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat
      481 cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac
      541 caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc
      601 caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga
      661 aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca
      721 ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt
      781 cccgtccgac ggtccggtta tgcagaaa
//
