LOCUS       sequence-106849-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Cow p53 (residues 1-82) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Daughdrill GW, Borcherds WM, Wu H
  TITLE     Disorder predictors also predict backbone dynamics for a family of 
            disordered proteins.
  JOURNAL   PLoS One. 2011;6(12):e29207. doi: 10.1371/journal.pone.0029207. Epub
            2011 Dec 15.
  PUBMED    22195023
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e29207. doi"; pages: " 
            10.1371/journal.pone.0029207. Epub 2011 Dec 15"
COMMENT     Sequence Label: pET28-p53 (Cow 1-82)
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(313..330)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(340..357)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             376..398
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    413..437
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(437..456)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(438..456)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(540..559)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        765..842
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(862..881)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cttctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtta
       61 agaggtggcg ggggcggggg tggctggggg tggggcggcg ggggctgagg gttcgggcat
      121 ttggggggct tcgtttgggc attcatctaa ccaggttgca acatcggtat aaggtaataa
      181 gtcatctact ggggcggaga gttctgaaga gagtaagttg ttttcgggta ataagttcca
      241 taagtctgag aaggtttctt gggataaagg gggttctaca tttaattcgg cttgggattc
      301 ttccatcata tggctgccgc gcggcaccag gccgctgctg tgatgatgat gatgatggct
      361 gctgcccatg gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt
      421 tatccgctca caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat
      481 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
      541 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
      601 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
      661 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
      721 cttcctaatg caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc
      781 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga
      841 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgt
//
